Nat Genet. 1999 Jan;21(1 Suppl):10-4.

Expression profiling using cDNA microarrays.

Duggan DJ, Bittner M, Chen Y, Meltzer P, Trent JM.

Source

Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

Abstract

cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3' expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent- or radioactively-labelled cDNAs. Here, we review technical aspects of cDNA microarrays, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining.

PMID:: 9915494; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Review

MeSH Terms

Substances

DNA, Complementary

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Libraries

LinkOut Holdings

Supplemental Content

Related citations

ESTs, cDNA microarrays, and gene expression profiling: tools for dissecting plant physiology and development. [Plant J. 2004]
ESTs, cDNA microarrays, and gene expression profiling: tools for dissecting plant physiology and development.
Alba R, Fei Z, Payton P, Liu Y, Moore SL, Debbie P, Cohn J, D'Ascenzo M, Gordon JS, Rose JK, et al. Plant J. 2004 Sep; 39(5):697-714.
Review Expression profiling in cancer using cDNA microarrays. [Electrophoresis. 1999]
Review Expression profiling in cancer using cDNA microarrays.
Khan J, Saal LH, Bittner ML, Chen Y, Trent JM, Meltzer PS. Electrophoresis. 1999 Feb; 20(2):223-9.
Profiling human gene expression with cDNA microarrays. [Curr Protoc Hum Genet. 2001]
Profiling human gene expression with cDNA microarrays.
Jiang Y, Lueders J, Glatfelter A, Gooden C, Bittner M. Curr Protoc Hum Genet. 2001 May; Chapter 11:Unit 11.3.
Review New experimental and computational approaches to the analysis of gene expression. [Acta Biochim Pol. 1998]
Review New experimental and computational approaches to the analysis of gene expression.
Rafalski JA, Hanafey M, Miao GH, Ching A, Lee JM, Dolan M, Tingey S. Acta Biochim Pol. 1998; 45(4):929-34.
Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays. [J Exp Bot. 2002]
Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays.
Aharoni A, O'Connell AP. J Exp Bot. 2002 Oct; 53(377):2073-87.

See reviews... See all...

Cited by over 100 PubMed Central articles

Gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow. [J Periodontal Implant Sci. 2011]
Gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow.
Kim SH, Kim YS, Lee SY, Kim KH, Lee YM, Kim WK, Lee YK. J Periodontal Implant Sci. 2011 Aug; 41(4):192-200. Epub 2011 Aug 31.
Transcriptome analysis of the effects of gomisin a on the recovery of carbon tetrachloride-induced damage in rat liver. [Lab Anim Res. 2011]
Transcriptome analysis of the effects of gomisin a on the recovery of carbon tetrachloride-induced damage in rat liver.
Choi YM, Choi IS, Lee SM, Hwang DY, Choi YW, Park YH. Lab Anim Res. 2011 Jun; 27(2):161-9. Epub 2011 Jun 22.
Preprocessing differential methylation hybridization microarray data. [BioData Min. 2011]
Preprocessing differential methylation hybridization microarray data.
Sun S, Huang YW, Yan PS, Huang TH, Lin S. BioData Min. 2011 May 16; 4:13. Epub 2011 May 16.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

Expression profiling using cDNA microarrays.
Expression profiling using cDNA microarrays.
Nat Genet. 1999 Jan ;21(1 Suppl):10-4.

PubMed
Complete gene map of the plastid-like DNA of the malaria parasite Plasmodium fal...
Complete gene map of the plastid-like DNA of the malaria parasite Plasmodium falciparum.
J Mol Biol. 1996 Aug 16 ;261(2):155-72.

PubMed
Pfam: a comprehensive database of protein domain families based on seed alignmen...
Pfam: a comprehensive database of protein domain families based on seed alignments.
Proteins. 1997 Jul ;28(3):405-20.

PubMed
Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.
Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.
Science. 1995 Jul 28 ;269(5223):496-512.

PubMed
Cytoplasmic incompatibility in Drosophila simulans: dynamics and parameter estim...
Cytoplasmic incompatibility in Drosophila simulans: dynamics and parameter estimates from natural populations.
Genetics. 1995 Aug ;140(4):1319-38.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: