Clin Chem. 1979 Jul;25(7):1317-9.

New micro-turbidimetric method for determination of protein in cerebrospinal fluid and urine.

Abstract

We report a new micro-scale (0.1-mL sample) turbidimetric method for determination of protein by use of benzethonium chloride in alkali. The method is highly specific for protein, has a higher sensitivity than the classic method of Lowry et al., and shows satisfactory reproducibility and recovery. The turbidity produced in our method is the same for albumin and gamma-globulin and is more stable than in Meulemans' method (in which sulfosalicylic acid is used) or in the method of Bossak et al. (in which trichloracetic acid is used). In contrast to Pesce and Strande's method, there is no manipulative loss of protein.

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[Turbidimetric protein determination with benzethonium chloride and sulfosalicylic acid]. [Z Med Lab Diagn. 1986]
[Turbidimetric protein determination with benzethonium chloride and sulfosalicylic acid].
Lehmitz R. Z Med Lab Diagn. 1986; 27(2):89-91.
[Micro-Turbidimetric method for determination of protein in cerebrospinal fluid and urine (author's transl)]. [Rinsho Byori. 1981]
[Micro-Turbidimetric method for determination of protein in cerebrospinal fluid and urine (author's transl)].
Iwata J, Nishikaze O. Rinsho Byori. 1981 May; 29(5):497-501.
[Valve of the use of benzethonium chloride for turbidimetric analysis of cerebrospinal fluid proteins with an automatic analyzer]. [Ann Biol Clin (Paris). 1983]
[Valve of the use of benzethonium chloride for turbidimetric analysis of cerebrospinal fluid proteins with an automatic analyzer].
Flachaire E, Later R, Caudie C, Quincy C. Ann Biol Clin (Paris). 1983; 41(1):39-44.
Review The quantitation and fractionation of proteins in cerebrospinal fluid. [Am J Med Technol. 1982]
Review The quantitation and fractionation of proteins in cerebrospinal fluid.
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Review The use and limitations of laser nephelometry.
Bergmann KC, Crisci CD, Oehling A. Allergol Immunopathol (Madr). 1981 Jan-Feb; 9(1):55-62.

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