J Clin Chem Clin Biochem. 1977 Mar;15(3):131-7.

Quantitative determination of creatine kinase isoenzyme catalytic concentrations in serum using immunological methods.

Würzburg U, Hennrich N, Orth HD, Lang H, Prellwitz W, Neumeier D, Knedel M, Rick W.

Abstract

For the determination of creatine kinase isoenzyme catalytic concentrations in serum two methods based on immunological reactions are presented: One method uses inhibiting antibodies, which selectively block the activity of creatine kinase M subunits ("Inhibition Test"). This test is used for routine measurements of creatine kinase MB catalytic concentration; Another method uses precipitating antibodies, which allows a quantitative differentiation of creatine kinase isoenzymes MM, MB and BB ("Precipitation Test"). This test is used as a control for the Inhibition Test for the possible presence of creatine kinase BB activities in doubtful cases. Procedures, specificity, correlation and application of these methods are discussed.

PMID:: 859004; [PubMed - indexed for MEDLINE]

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Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity. Activity kinetics and diagnostic significance in myocardial infarction. [Clin Chim Acta. 1976]
Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity. Activity kinetics and diagnostic significance in myocardial infarction.
Neumeier D, Prellwitz W, Würzburg U, Brundobler M, Olbermann M, Just H-J, Knedel M, Lang H. Clin Chim Acta. 1976 Dec; 73(3):445-51.
[Determination of creatine kinase-MB in serum using inhibiting antibodies (author's transl)]. [Klin Wochenschr. 1976]
[Determination of creatine kinase-MB in serum using inhibiting antibodies (author's transl)].
Würzburg U, Hennrich N, Lang H, Prellwitz W, Neumeier D, Knedel M. Klin Wochenschr. 1976 Apr 15; 54(8):357-60.
[Creatine kinase isoenzymes: idiopathic occurrence of creatine kinase BB activities in patient serum (author's transl)]. [Klin Wochenschr. 1978]
[Creatine kinase isoenzymes: idiopathic occurrence of creatine kinase BB activities in patient serum (author's transl)].
Lang H, Würzburg U, Neumeier D, Knedel M, Prellwitz W, Kattermann R, Schlebusch H, Schürmann J. Klin Wochenschr. 1978 Jul 1; 56(13):641-6.
Creatine kinase isoenzyme MB determination on the ACA: dependence on serum matrix and other effectors. [J Clin Chem Clin Biochem. 1984]
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Golf SW, Kaul-Kunz C, Róka L. J Clin Chem Clin Biochem. 1984 Nov; 22(11):751-8.
Creatine kinase isoenzyme variants in human serum. [Clin Chem. 1978]
Creatine kinase isoenzyme variants in human serum.
Ljungdahl L, Gerhardt W. Clin Chem. 1978 May; 24(5):832-4.

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Quantitative determination of creatine kinase isoenzyme catalytic concentrations in serum using immunological methods.

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