Ann Biomed Eng. 1993;21(1):77-83.

Design and construction of a linear shear stress flow chamber.

Usami S, Chen HH, Zhao Y, Chien S, Skalak R.

Source

Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.

Abstract

A new parallel plate flow chamber that has a linear variation of shear stress, starting from a predetermined maximum value at the entrance and falling to zero at the exit, has been designed and tested. This is in contrast to the usual rectangular channel plan which produces a constant shear stress over the entire length. The new design is based on the theory of Hele-Shaw flow between parallel plates. To verify the efficacy of the flow channel, the effect of fluid shear stress on platelet adhesion to a fibrinogen-coated glass surface was tested. The percentage of attached platelets after 5 min of shear stress is shown to be a function of shear stress. With this new flow chamber, cell-cell interactions can be studied efficiently over a wide range of shear stress using a single run at constant discharge.

PMID:: 8434823; [PubMed - indexed for MEDLINE]

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Fibrinogen

Grant Support

HL 43026/HL/NHLBI NIH HHS/United States

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