Clin Chim Acta. 1980 Feb 28;101(2-3):299-303.

Determination of uric acid with uricase and peroxidase.

Abstract

A reliable method for the determination of uric acid in plasma or serum is described. The hydrogen peroxidase developed in the uricase reaction is used, together with peroxidase, for the coupling of sulphonated dichlorophenol and 4-aminoantipyine to a red dye. The difference in absorbance at 515 nm before and after addition of uricase is measured. Of the components tested for interference some gave rise to falsely lowered values. Reagents are cheap and the high molecular absorption coefficient of the red dye permits the use of small sample volumes.

PMID:: 7357749; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Molecular Biology Databases

HYDROGEN PEROXIDE - HSDB

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Determination of uric acid on continuous-flow (AutoAnalyzer II and SMA) systems with a uricase/phenol/4-aminophenazone color test. [Clin Chem. 1978]
Determination of uric acid on continuous-flow (AutoAnalyzer II and SMA) systems with a uricase/phenol/4-aminophenazone color test.
Klose S, Stoltz M, Munz E, Portenhauser R. Clin Chem. 1978 Feb; 24(2):250-5.
New enzymatic method for serum uric acid at 500 nm. [Clin Chem. 1978]
New enzymatic method for serum uric acid at 500 nm.
Trivedi RC, Rebar L, Berta E, Stong L. Clin Chem. 1978 Nov; 24(11):1908-11.
Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method. [J Zhejiang Univ Sci B. 2006]
Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method.
Liao F, Zhao YS, Zhao LN, Tao J, Zhu XY, Liu L. J Zhejiang Univ Sci B. 2006 Jun; 7(6):497-502.
Review Technical bulletin No. 31. Determination of uric acid in blood and in urine. [Ann Clin Biochem. 1974]
Review Technical bulletin No. 31. Determination of uric acid in blood and in urine.
Watts RW. Ann Clin Biochem. 1974 Jul; 11(4):103-11.
Review [How do we set the standard value of serum uric acid levels?]. [Nihon Rinsho. 2008]
Review [How do we set the standard value of serum uric acid levels?].
Hamada T, Igawa O, Shigemasa C, Hisatome I. Nihon Rinsho. 2008 Apr; 66(4):723-7.

See reviews... See all...

Cited by 3 PubMed Central articles

Hyperuricemia in obese children and adolescents: the relationship with metabolic syndrome. [Pediatr Rep. 2010]
Hyperuricemia in obese children and adolescents: the relationship with metabolic syndrome.
Tang L, Kubota M, Nagai A, Mamemoto K, Tokuda M. Pediatr Rep. 2010 Jun 18; 2(1):e12. Epub 2010 Jun 18.
Effect of Carnitine and herbal mixture extract on obesity induced by high fat diet in rats. [Diabetol Metab Syndr. 2009]
Effect of Carnitine and herbal mixture extract on obesity induced by high fat diet in rats.
Amin KA, Nagy MA. Diabetol Metab Syndr. 2009 Oct 16; 1(1):17. Epub 2009 Oct 16.
Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method. [J Zhejiang Univ Sci B. 2006]
Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method.
Liao F, Zhao YS, Zhao LN, Tao J, Zhu XY, Liu L. J Zhejiang Univ Sci B. 2006 Jun; 7(6):497-502.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Determination of uric acid with uricase and peroxidase.
Determination of uric acid with uricase and peroxidase.
Clin Chim Acta. 1980 Feb 28 ;101(2-3):299-303.

PubMed
Determination of malonaldehyde precursor in tissues by thiobarbituric acid test.
Determination of malonaldehyde precursor in tissues by thiobarbituric acid test.
Anal Biochem. 1978 May ;86(1):271-8.

PubMed
Obesity, alcohol consumption and blood pressure in Australian men and women. The...
Obesity, alcohol consumption and blood pressure in Australian men and women. The National Heart Foundation of Australia Risk Factor Prevalence Study.
J Hypertens. 1984 Feb ;2(1):85-91.

PubMed
Iron absorption from infant milk formula and the optimal level of iron supplemen...
Iron absorption from infant milk formula and the optimal level of iron supplementation.
Acta Paediatr Scand. 1977 Nov ;66(6):719-22.

PubMed
Iron balance and requirements in infancy.
Iron balance and requirements in infancy.
Pediatrics. 1969 Jan ;43(1):134-42.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: