Gene. 1984 Dec;32(3):487-92.

Cloning gene ura5 for the orotidylic acid pyrophosphorylase of the filamentous fungus Podospora anserina: transformation of protoplasts.

Bégueret J, Razanamparany V, Perrot M, Barreau C.

Abstract

From a genomic library of the filamentous fungus Podospora anserina, we have cloned a 4.9-kb fragment which complements an Escherichia coli mutant strain deficient for orotidylic acid pyrophosphorylase (pyrE gene). The recombinant plasmid pPAura5 also transforms to prototrophy a mutant strain of P. anserina carrying a mutation in the ura5 gene and lacking OMPppase activity.

PMID:: 6530144; [PubMed - indexed for MEDLINE]

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Cloning gene ura5 for the orotidylic acid pyrophosphorylase of the filamentous f...
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Cloning gene ura5 for the orotidylic acid pyrophosphorylase of the filamentous fungus Podospora anserina: transformation of protoplasts.

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