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    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4149-53.

    Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA.

    Hirashima A, Wang S, Inouye M.

    Abstract

    A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of the purified mRNA. The product of the cell-free system was identified as the specific lipoprotein by immunoprecipitation and by peptide mapping.

    PMID:
    4610570
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC434347
    Free PMC Article

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