Clin Chem. 1979 Aug;25(8):1388-93.

Spectrophotometric measurement of carboxyhemoglobin and methemoglobin in blood.

Rodkey FL, Hill TA, Pitts LL, Robertson RF.

Abstract

This paper describes separate spectrophotometric procedures for rapidly measuring carboxyhemoglobin (COHb) and methemoglobin (MetHb) in blood. Absorbance measurements are made in the Soret region at a blood dilution near 1000-fold. For COHb estimation, the diluent contains sodium hydrosulfite, providing the two-component system COHb--Hb for absorbance measurements at 420 and 432 nm. The NA2S2O4 effectively prevents dissociation of COHb by oxygen. For MetHb estimation, the diluent contains KCN and carbon monoxide, providing the two-component system COHb--CNMetHb. Absorbance measurements are made at 420 nm, before and after addiely analyzed for the total of all MetHb forms present. Each procedure requires about 3 muL of blood and can be applied to human or animal blood. Results obtained by the present methods are in satisfactory agreement with currently accepted procedures, which require much larger samples or more elaborate equipment.

PMID:: 455674; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

A simple spectrophotometry for determination of carboxyhemoglobin in blood. [J Forensic Sci. 1982]
A simple spectrophotometry for determination of carboxyhemoglobin in blood.
Katsumata Y, Aoki M, Sato K, Suzuki O, Oya M, Yada S. J Forensic Sci. 1982 Oct; 27(4):928-34.
Methemoglobin in blood as determined by double-wavelength spectrophotometry. [Clin Chem. 1982]
Methemoglobin in blood as determined by double-wavelength spectrophotometry.
Sakata M, Yoshida A, Haga M. Clin Chem. 1982 Mar; 28(3):508-11.
Determination of methemoglobin and carboxyhemoglobin in blood by rapid colorimetry. [Biol Pharm Bull. 1997]
Determination of methemoglobin and carboxyhemoglobin in blood by rapid colorimetry.
Matsuoka T. Biol Pharm Bull. 1997 Nov; 20(11):1208-11.
Validation of a spectrophotometric method for quantification of carboxyhemoglobin. [J Anal Toxicol. 2009]
Validation of a spectrophotometric method for quantification of carboxyhemoglobin.
Luchini PD, Leyton JF, Strombech Mde L, Ponce JC, Jesus MG, Leyton V. J Anal Toxicol. 2009 Oct; 33(8):540-4.
Review Spectrophotometry of hemoglobin and hemoglobin derivatives. [Adv Clin Chem. 1983]
Review Spectrophotometry of hemoglobin and hemoglobin derivatives.
van Kampen EJ, Zijlstra WG. Adv Clin Chem. 1983; 23:199-257.

See reviews... See all...

Cited by 4 PubMed Central articles

NF-κB inducing kinase, NIK mediates cigarette smoke/TNFα-induced histone acetylation and inflammation through differential activation of IKKs. [PLoS One. 2011]
NF-κB inducing kinase, NIK mediates cigarette smoke/TNFα-induced histone acetylation and inflammation through differential activation of IKKs.
Chung S, Sundar IK, Hwang JW, Yull FE, Blackwell TS, Kinnula VL, Bulger M, Yao H, Rahman I. PLoS One. 2011; 6(8):e23488. Epub 2011 Aug 24.
The effects of second-hand smoke on biological processes important in atherogenesis. [BMC Cardiovasc Disord. 2007]
The effects of second-hand smoke on biological processes important in atherogenesis.
Yuan H, Wong LS, Bhattacharya M, Ma C, Zafarani M, Yao M, Schneider M, Pitas RE, Martins-Green M. BMC Cardiovasc Disord. 2007 Jan 8; 7:1. Epub 2007 Jan 8.
Nitric oxide is consumed, rather than conserved, by reaction with oxyhemoglobin under physiological conditions. [Proc Natl Acad Sci U S A. 2002]
Nitric oxide is consumed, rather than conserved, by reaction with oxyhemoglobin under physiological conditions.
Joshi MS, Ferguson TB Jr, Han TH, Hyduke DR, Liao JC, Rassaf T, Bryan N, Feelisch M, Lancaster JR Jr. Proc Natl Acad Sci U S A. 2002 Aug 6; 99(16):10341-6. Epub 2002 Jul 17.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Spectrophotometric measurement of carboxyhemoglobin and methemoglobin in blood.
Spectrophotometric measurement of carboxyhemoglobin and methemoglobin in blood.
Clin Chem. 1979 Aug ;25(8):1388-93.

PubMed
A new subunit influenza vaccine: acceptability compared with standard vaccines a...
A new subunit influenza vaccine: acceptability compared with standard vaccines and effect of dose on antigenicity.
J Infect Dis. 1972 Jun ;125(6):656-64.

PubMed
[Alveolar hydatidosis (ecchinococosis) diagnosed by liver puncture biopsy (autho...
[Alveolar hydatidosis (ecchinococosis) diagnosed by liver puncture biopsy (author's transl)].
Cas Lek Cesk. 1979 Apr 13 ;118(15):472-5.

PubMed
Construction and properties of Escherichia coli strains exhibiting -complementa...
Construction and properties of Escherichia coli strains exhibiting -complementation of -galactosidase fragments in vivo.
J Bacteriol. 1972 Apr ;110(1):171-8.

PubMed
Increased near-ultraviolet induced DNA fragmentation in xeroderma pigmentosum va...
Increased near-ultraviolet induced DNA fragmentation in xeroderma pigmentosum variants.
Biochem Biophys Res Commun. 1979 Apr 13 ;87(3):802-10.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: