QTLs Related to Rice Callus Regeneration Ability: Localization and Effect Verification of qPRR3

Jiemin Wu; Xinlei Chang; Chuanhong Li; Zhaoyang Zhang; Jianguo Zhang; Changxi Yin; Weihua Ma; Hao Chen; Fei Zhou; Yongjun Lin

doi:10.3390/cells11244125

QTLs Related to Rice Callus Regeneration Ability: Localization and Effect Verification of qPRR3

Cells. 2022 Dec 19;11(24):4125. doi: 10.3390/cells11244125.

Authors

Jiemin Wu^{1

2}, Xinlei Chang^{1

2}, Chuanhong Li^{1

2}, Zhaoyang Zhang^{1

2}, Jianguo Zhang^{1

2}, Changxi Yin^{1

3}, Weihua Ma^{1

3}, Hao Chen^{1

2}, Fei Zhou^{1

2}, Yongjun Lin^{1

2}

Affiliations

¹ National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research, Wuhan 430070, China.
² College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
³ College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Abstract

Mature and efficient tissue culture systems are already available for most japonica rice varieties (Oryza sativa ssp. geng). However, it remains challenging to regenerate the majority of indica rice varieties (Oryza sativa ssp. xian). In this study, quantitative trait loci (QTLs) associated with rice callus regeneration ability were identified based on the plant regeneration rate (PRR) and total green plant rate (TGPR) of the 93-11 × Nip recombinant inbred line population. Significant positive correlations were found between PRR and TGPR. A total of three QTLs (one for PRR and two for TGPR) were identified. qPRR3 (located on chromosome 3) was detected for both traits, which could explain 13.40% and 17.07% of the phenotypic variations of PRR and TGPR, respectively. Subsequently, the effect of qPRR3 on callus regeneration ability was validated by cryptographically tagged near-isogenic lines (NILs), and the QTL was narrowed to an interval of approximately 160 kb. The anatomical structure observation of the regenerated callus of the NILs revealed that qPRR3 can improve the callus regeneration ability by promoting the regeneration of shoots.

Keywords: QTL; callus; callus regeneration ability; indica rice; plant regeneration rate; recombinant inbred line; tissue culture; total green plant rate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping
Oryza* / genetics
Phenotype
Quantitative Trait Loci* / genetics

Grants and funding

This research was funded by the National Program of Transgenic Variety Development of China (grant number 2016ZX08001-001) and the National Program of Science Research and Development of China (grant number 2016YFD0100600).