Eur J Immunol. 1987 Oct;17(10):1411-6.

Production of hybridoma growth factor by human monocytes.

Aarden LA, De Groot ER, Schaap OL, Lansdorp PM.

Source

Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.

Abstract

Human mononuclear leukocytes produce a growth factor (HGF) for hybridoma and plasmacytoma cells. HGF has recently been proven to be identical to IFN-beta 2, 26-kDa protein and BSF-2. HGF can be quantitated in a proliferation assay with the HGF-dependent hybridoma cell line B13.29. By selection of an extremely sensitive variant of this cell line, we were able to measure HGF production of single cells. Limiting dilution analysis of the producing cells in combination with size, density and adherence characteristics showed that HGF is produced by monocytes and not by lymphocytes. There was no need for the monocytes to be stimulated but the cells did require the presence of serum. This serum requirement could be met by purified bovine serum albumin, but not by other proteins like ovalbumin or human gamma-globulin. HGF production in vitro by monocytes starts after 2 h of incubation and is completed within 24 h.

PMID:: 3500054; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

John Wiley & Sons, Inc.

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Interleukin 1 and poly(rI).poly(rC) induce production of a hybridoma growth factor by human fibroblasts. [Eur J Immunol. 1987]
Interleukin 1 and poly(rI).poly(rC) induce production of a hybridoma growth factor by human fibroblasts.
Van Damme J, Cayphas S, Opdenakker G, Billiau A, Van Snick J. Eur J Immunol. 1987 Jan; 17(1):1-7.
Role of the macrophage-derived hybridoma growth factor in the in vitro and in vivo proliferation of newly formed B cell hybridomas. [J Immunol. 1987]
Role of the macrophage-derived hybridoma growth factor in the in vitro and in vivo proliferation of newly formed B cell hybridomas.
Bazin R, Lemieux R. J Immunol. 1987 Aug 1; 139(3):780-7.
A T-helper cell x Molt4 human hybridoma constitutively producing B-cell stimulatory and inhibitory factors. [Lymphokine Res. 1986]
A T-helper cell x Molt4 human hybridoma constitutively producing B-cell stimulatory and inhibitory factors.
Rosén A, Uggla C, Szigeti R, Kallin B, Lindqvist C, Zeuthen J. Lymphokine Res. 1986 Summer; 5(3):185-204.
Induction of hybridoma growth factor (HGF), identical to IL-6, in human fibroblasts by IL-1: use of HGF activity in specific and sensitive biological assays for IL-1 and IL-6. [Dev Biol Stand. 1988]
Induction of hybridoma growth factor (HGF), identical to IL-6, in human fibroblasts by IL-1: use of HGF activity in specific and sensitive biological assays for IL-1 and IL-6.
Van Damme J, Van Snick J. Dev Biol Stand. 1988; 69:31-8.
Induction of met proto-oncogene (hepatocyte growth factor receptor) expression during human monocyte-macrophage differentiation. [Cell Growth Differ. 1996]
Induction of met proto-oncogene (hepatocyte growth factor receptor) expression during human monocyte-macrophage differentiation.
Chen Q, DeFrances MC, Zarnegar R. Cell Growth Differ. 1996 Jun; 7(6):821-32.

See reviews... See all...

Cited by over 100 PubMed Central articles

The role of aldosterone in mediating the dependence of angiotensin hypertension on IL-6. [Am J Physiol Regul Integr Comp...]
The role of aldosterone in mediating the dependence of angiotensin hypertension on IL-6.
Sturgis LC, Cannon JG, Schreihofer DA, Brands MW. Am J Physiol Regul Integr Comp Physiol. 2009 Dec; 297(6):R1742-8. Epub 2009 Oct 7.
Raised intrathecal levels of APRIL and BAFF in patients with systemic lupus erythematosus: relationship to neuropsychiatric symptoms. [Arthritis Res Ther. 2008]
Raised intrathecal levels of APRIL and BAFF in patients with systemic lupus erythematosus: relationship to neuropsychiatric symptoms.
George-Chandy A, Trysberg E, Eriksson K. Arthritis Res Ther. 2008; 10(4):R97. Epub 2008 Aug 22.
Dimeric and polymeric IgA, but not monomeric IgA, enhance the production of IL-6 by human renal mesangial cells. [Mediators Inflamm. 1996]
Dimeric and polymeric IgA, but not monomeric IgA, enhance the production of IL-6 by human renal mesangial cells.
Reterink TJ, Schroeijers WE, Es LA, Daha MR. Mediators Inflamm. 1996; 5(3):191-5.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Production of hybridoma growth factor by human monocytes.
Production of hybridoma growth factor by human monocytes.
Eur J Immunol. 1987 Oct ;17(10):1411-6.

PubMed
Immune response in the mutant diabetic C57BL/Ks-dt+ mouse. Discrepancies between...
Immune response in the mutant diabetic C57BL/Ks-dt+ mouse. Discrepancies between in vitro and in vivo immunological assays.
J Clin Invest. 1978 Feb ;61(2):243-50.

PubMed
Accuracy and precision of clinical estimation of leg length inequality and lumba...
Accuracy and precision of clinical estimation of leg length inequality and lumbar scoliosis: comparison of clinical and radiological measurements.
Int Disabil Stud. 1988 ;10(2):49-53.

PubMed
Arthritis in rats after systemic injection of streptococcal cells or cell walls.
Arthritis in rats after systemic injection of streptococcal cells or cell walls.
J Exp Med. 1977 Dec 1 ;146(6):1585-602.

PubMed
Transient osteoporosis: transient bone marrow edema?
Transient osteoporosis: transient bone marrow edema?
Radiology. 1988 Jun ;167(3):757-60.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: