Science. 1988 Aug 26;241(4869):1077-80.

A ligase-mediated gene detection technique.

Landegren U, Kaiser R, Sanders J, Hood L.

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Division of Biology, California Institute of Technology, Pasadena 91125.

Abstract

An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule. The two oligonucleotides are then joined covalently by the action of a DNA ligase, provided that the nucleotides at the junction are correctly base-paired. Thus single nucleotide substitutions can be distinguished. This strategy permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.

PMID:: 3413476; [PubMed - indexed for MEDLINE]

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A ligase-mediated gene detection technique.
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