Proc Natl Acad Sci U S A. 1987 Oct;84(19):6879-83.

Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor.

Source

Howard Hughes Medical Institute, Department of Medicine, University of California, San Francisco 94143.

Abstract

We have developed an approach for deriving and characterizing antigen-receptor (CD3/Ti) signal-transduction mutants. This strategy combines receptor-mediated growth inhibition and fluorescence-activated cell sorting with the Ca2+-indicator indo-1. Despite the expression of structurally normal CD3/Ti complexes, one such mutant (J.CaM1) fails to exhibit inositolphospholipid metabolism or Ca2+ mobilization in response to anti-CD3 or anti-Ti monoclonal antibodies and fails to produce lymphokines in response to these antibodies. Surprisingly, anti-Ti antibody retains its effectiveness as a stimulus for the down-regulation of CD3/Ti surface expression. These cells remain responsive to AIF-4, at least one anti-CD3 antibody, and some combinations of nonagonist anti-Ti and anti-CD3 antibodies. The mutation in J.CaM1 appears to lie in a proximal component of the signal-transduction apparatus.

PMID:: 3309950; [PubMed - indexed for MEDLINE]
PMCID:: PMC299188

Free PMC Article

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Function of a heterologous muscarinic receptor in T cell antigen receptor signal transduction mutants. [J Biol Chem. 1989]
Function of a heterologous muscarinic receptor in T cell antigen receptor signal transduction mutants.
Goldsmith MA, Desai DM, Schultz T, Weiss A. J Biol Chem. 1989 Oct 15; 264(29):17190-7.
At least two non-antigen-binding molecules are required for signal transduction by the T-cell antigen receptor. [Proc Natl Acad Sci U S A. 1988]
At least two non-antigen-binding molecules are required for signal transduction by the T-cell antigen receptor.
Goldsmith MA, Dazin PF, Weiss A. Proc Natl Acad Sci U S A. 1988 Nov; 85(22):8613-7.
Antigen receptor-mediated regulation of sustained polyphosphoinositide turnover in a human T cell line. Evidence for a receptor-regulated pathway for production of phosphatidylinositol 4,5-bisphosphate. [J Biol Chem. 1990]
Antigen receptor-mediated regulation of sustained polyphosphoinositide turnover in a human T cell line. Evidence for a receptor-regulated pathway for production of phosphatidylinositol 4,5-bisphosphate.
Inokuchi S, Imboden JB. J Biol Chem. 1990 Apr 15; 265(11):5983-9.
Review Ca2+ signaling in T-lymphocytes. [Crit Rev Immunol. 1998]
Review Ca2+ signaling in T-lymphocytes.
Guse AH. Crit Rev Immunol. 1998; 18(5):419-48.
Review Generation and analysis of a T-lymphocyte somatic mutant for studying molecular aspects of signal transduction by the antigen receptor. [Ann N Y Acad Sci. 1988]
Review Generation and analysis of a T-lymphocyte somatic mutant for studying molecular aspects of signal transduction by the antigen receptor.
Goldsmith MA, Weiss A. Ann N Y Acad Sci. 1988; 546:91-103.

See reviews... See all...

Cited by 34 PubMed Central articles

Basal LAT-diacylglycerol-RasGRP1 signals in T cells maintain TCRα gene expression. [PLoS One. 2011]
Basal LAT-diacylglycerol-RasGRP1 signals in T cells maintain TCRα gene expression.
Markegard E, Trager E, Yang CW, Zhang W, Weiss A, Roose JP. PLoS One. 2011; 6(9):e25540. Epub 2011 Sep 26.
2B4 utilizes ITAM-containing receptor complexes to initiate intracellular signaling and cytolysis. [Mol Immunol. 2011]
2B4 utilizes ITAM-containing receptor complexes to initiate intracellular signaling and cytolysis.
Bida AT, Upshaw Neff JL, Dick CJ, Schoon RA, Brickshawana A, Chini CC, Billadeau DD. Mol Immunol. 2011 May; 48(9-10):1149-59. Epub 2011 Mar 25.
Herpes simplex virus requires VP11/12 to induce phosphorylation of the activation loop tyrosine (Y394) of the Src family kinase Lck in T lymphocytes. [J Virol. 2009]
Herpes simplex virus requires VP11/12 to induce phosphorylation of the activation loop tyrosine (Y394) of the Src family kinase Lck in T lymphocytes.
Wagner MJ, Smiley JR. J Virol. 2009 Dec; 83(23):12452-61. Epub 2009 Sep 23.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Isolation and characterization of a T-lymphocyte somatic mutant with altered sig...
Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor.
Proc Natl Acad Sci U S A. 1987 Oct ;84(19):6879-83.

PubMed
One strand equivalent of the Escherichia coli genome is transcribed: complexity ...
One strand equivalent of the Escherichia coli genome is transcribed: complexity and abundance classes of mRNA.
Science. 1977 Aug 5 ;197(4303):582-5.

PubMed
Regulation of 70-kilodalton heat-shock-like messenger ribonucleic acid in vitro ...
Regulation of 70-kilodalton heat-shock-like messenger ribonucleic acid in vitro and in vivo by prolactin.
Mol Endocrinol. 1987 Jun ;1(6):430-4.

PubMed
Commitment to the mitotic cell cycle in yeast in relation to meiosis.
Commitment to the mitotic cell cycle in yeast in relation to meiosis.
Exp Cell Res. 1977 Mar 15 ;105(2):245-52.

PubMed
Amino acid side chain parameters for correlation studies in biology and pharmaco...
Amino acid side chain parameters for correlation studies in biology and pharmacology.
Int J Pept Protein Res. 1988 Oct ;32(4):269-78.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor.

Source

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Research Materials

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 34 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information