Science. 1977 Aug 5;197(4303):582-5.

One strand equivalent of the Escherichia coli genome is transcribed: complexity and abundance classes of mRNA.

Abstract

DNA-RNA hybridization experiments show that essentially all of the genomic information is transcribed. High, intermediate, and rare abundance classes of messenger RNA (mRNA) are present, and their estimated complexities are equal to about 240, 1300, and 700 average-sized mRNA species, respectively. The high abundance mRNA species are present, an average, two to three copies per cell and constitute about 95 percent of the mRNA mass. Intermediate abundance mRNA species are present, on average, about once per 35 cells. The relative abundance and complexity of these mRNA classes correspond well with previous respective measurements on protein. Rare RNA species are thought to represent maximally repressed genes. Analysis of RNA synthesized in vitro by isolated nucleoids (chromosomes) suggests that sense and nonsense sequences are extensively interspersed on a given strand of the DNA.

PMID:: 327551; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

The expression of a plant genome in hnRNA and mRNA. [Nucleic Acids Res. 1979]
The expression of a plant genome in hnRNA and mRNA.
Kiper M, Bartels D, Herzfeld F, Richter G. Nucleic Acids Res. 1979; 6(5):1961-78.
In vitro transcription of the tryptophan operon in isolated bacterial nucleoids. [Mol Gen Genet. 1976]
In vitro transcription of the tryptophan operon in isolated bacterial nucleoids.
Ishii S, Imamoto F. Mol Gen Genet. 1976 Nov 17; 148(3):295-305.
Adenovirus type 2 early nuclear and mRNA: kinetic estimation of l anf r DNA strand fractions complementary to different abundance classes of viral RNA. [J Virol. 1977]
Adenovirus type 2 early nuclear and mRNA: kinetic estimation of l anf r DNA strand fractions complementary to different abundance classes of viral RNA.
Wold WS, Green M, Brackmann KH, Devine C, Cartas MA. J Virol. 1977 Sep; 23(3):616-25.
A mutant of Escherichia coli with a new, highly efficient promoter for the lactose operon. [Proc Natl Acad Sci U S A. 1973]
A mutant of Escherichia coli with a new, highly efficient promoter for the lactose operon.
Bruenn J, Hollingsworth H. Proc Natl Acad Sci U S A. 1973 Dec; 70(12):3693-7.
Review Gene manipulation: progress and prospects. [Symp Soc Dev Biol. 1974]
Review Gene manipulation: progress and prospects.
Signer E. Symp Soc Dev Biol. 1974; 30(0):217-41.

See reviews... See all...

Cited by 8 PubMed Central articles

Innovative approach for transcriptomic analysis of obligate intracellular pathogen: selective capture of transcribed sequences of Ehrlichia ruminantium. [BMC Mol Biol. 2009]
Innovative approach for transcriptomic analysis of obligate intracellular pathogen: selective capture of transcribed sequences of Ehrlichia ruminantium.
Emboulé L, Daigle F, Meyer DF, Mari B, Pinarello V, Sheikboudou C, Magnone V, Frutos R, Viari A, Barbry P, et al. BMC Mol Biol. 2009 Dec 24; 10:111. Epub 2009 Dec 24.
Review The mammalian transcriptome and the function of non-coding DNA sequences. [Genome Biol. 2004]
Review The mammalian transcriptome and the function of non-coding DNA sequences.
Shabalina SA, Spiridonov NA. Genome Biol. 2004; 5(4):105. Epub 2004 Mar 25.
Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS). [Proc Natl Acad Sci U S A. 1999]
Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS).
Graham JE, Clark-Curtiss JE. Proc Natl Acad Sci U S A. 1999 Sep 28; 96(20):11554-9.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

One strand equivalent of the Escherichia coli genome is transcribed: complexity ...
One strand equivalent of the Escherichia coli genome is transcribed: complexity and abundance classes of mRNA.
Science. 1977 Aug 5 ;197(4303):582-5.

PubMed
Regulation of 70-kilodalton heat-shock-like messenger ribonucleic acid in vitro ...
Regulation of 70-kilodalton heat-shock-like messenger ribonucleic acid in vitro and in vivo by prolactin.
Mol Endocrinol. 1987 Jun ;1(6):430-4.

PubMed
Commitment to the mitotic cell cycle in yeast in relation to meiosis.
Commitment to the mitotic cell cycle in yeast in relation to meiosis.
Exp Cell Res. 1977 Mar 15 ;105(2):245-52.

PubMed
Amino acid side chain parameters for correlation studies in biology and pharmaco...
Amino acid side chain parameters for correlation studies in biology and pharmacology.
Int J Pept Protein Res. 1988 Oct ;32(4):269-78.

PubMed
Plasmid transfer in Haemophilus influenzae.
Plasmid transfer in Haemophilus influenzae.
J Bacteriol. 1979 Aug ;139(2):520-9.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

One strand equivalent of the Escherichia coli genome is transcribed: complexity and abundance classes of mRNA.

Abstract

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 8 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information