Warning: The NCBI web site requires JavaScript to function. more...
Generate a file for use with external citation management software.
Genentech, Inc., South San Francisco, CA 94080.
Human and animal proteins of therapeutic value can be produced in E. coli, a gram-negative bacteria. Endotoxin, a cellular component, is reported to have clinically significant health effects. Operations--including culturing the microbe, separating solids by centrifugation, and mixing/homogenizing--had associated endotoxin levels ranging from 0.07 ng/m3 to 12.8 ng/m3. Utilizing a 10-fold safety factor under the threshold where clinically significant changes can be detected, an action level of 30 mg/m3 for large scale operations involving the use of E. coli was established. Operations conducted without engineering controls had maximum airborne endotoxin levels of 1812 ng/m3.
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on