Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1

Cristobal Espinosa Ruiz; Simona Manuguerra; Alberto Cuesta; Andrea Santulli; Concetta M Messina

doi:10.3390/ijerph16030474

Oxidative Stress, Induced by Sub-Lethal Doses of BDE 209, Promotes Energy Management and Cell Cycle Modulation in the Marine Fish Cell Line SAF-1

Int J Environ Res Public Health. 2019 Feb 6;16(3):474. doi: 10.3390/ijerph16030474.

Authors

Cristobal Espinosa Ruiz¹, Simona Manuguerra², Alberto Cuesta³, Andrea Santulli^{4

5}, Concetta M Messina⁶

Affiliations

¹ Laboratory of Marine biochemistry and ecotoxicology, Department of Earth and Marine Science DISTEM, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy. cristobal.espinosaruiz@unipa.it.
² Laboratory of Marine biochemistry and ecotoxicology, Department of Earth and Marine Science DISTEM, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy. simona.manuguerra@unipa.it.
³ Fish Innate Immune System Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain. alcuesta@um.es.
⁴ Laboratory of Marine biochemistry and ecotoxicology, Department of Earth and Marine Science DISTEM, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy. andrea.santulli@unipa.it.
⁵ Marine Biology Institute, Consorzio Universitario della Provincia di Trapani, Via Barlotta 4, 91100 Trapani, Italy. andrea.santulli@unipa.it.
⁶ Laboratory of Marine biochemistry and ecotoxicology, Department of Earth and Marine Science DISTEM, University of Palermo, Via Barlotta 4, 91100 Trapani, Italy. concetta.messina@unipa.it.

Abstract

The effects of sub-lethal doses of polybrominated diphenyl ether (PBDE)-209 in terms of toxicity, oxidative stress, and biomarkers were evaluated in the Sparus aurata fibroblast cell line (SAF-1). Vitality and oxidative stress status were studied after incubation with PBDE for 72 h. Concomitantly, the quantification of proteins related to cell cycle and DNA repair (p53), cell proliferation (extracellular signal⁻regulated kinase 1 (ERK1)), energetic restriction (hypoxia-inducible factor 1 (HIF1)), and redox status (Nuclear factor erythroid 2⁻related factor 2 (NRF2)) was also determined after prolonged exposure (7⁻15 days) by immunoblotting. Our results demonstrated that rising concentrations of PBDEs exposure-induced oxidative stress, and that this event modulates different cell pathways related to cell cycle, cell signaling, and energetic balance in the long term, indicating the negative impact of sub-lethal dose exposure to cell homeostasis.

Keywords: PBDE-209; Sparus aurata fibroblast; biomarkers; oxidative stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aquatic Organisms / drug effects*
Cells, Cultured / drug effects*
Cytotoxins / adverse effects
Dose-Response Relationship, Drug
Fibroblasts / drug effects*
Halogenated Diphenyl Ethers / adverse effects*
Oxidation-Reduction / drug effects*
Oxidative Stress / drug effects*
Sea Bream / growth & development*

Substances

Cytotoxins
Halogenated Diphenyl Ethers