Science. 1989 Aug 11;245(4918):616-21.

Conserved folding in retroviral proteases: crystal structure of a synthetic HIV-1 protease.

Wlodawer A, Miller M, Jaskólski M, Sathyanarayana BK, Baldwin E, Weber IT, Selk LM, Clawson L, Schneider J, Kent SB.

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Crystallography Laboratory, NCI-Frederick Cancer Research Facility, MD 21701.

Abstract

The rational design of drugs that can inhibit the action of viral proteases depends on obtaining accurate structures of these enzymes. The crystal structure of chemically synthesized HIV-1 protease has been determined at 2.8 angstrom resolution (R factor of 0.184) with the use of a model based on the Rous sarcoma virus protease structure. In this enzymatically active protein, the cysteines were replaced by alpha-amino-n-butyric acid, a nongenetically coded amino acid. This structure, in which all 99 amino acids were located, differs in several important details from that reported previously by others. The interface between the identical subunits forming the active protease dimer is composed of four well-ordered beta strands from both the amino and carboxyl termini and residues 86 to 94 have a helical conformation. The observed arrangement of the dimer interface suggests possible designs for dimerization inhibitors.

PMID:: 2548279; [PubMed - indexed for MEDLINE]

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Structures reported by this article

Crystal Structure Of Monomeric M-Pmv Retroviral Protease

PDB: 3SQF

Source: Mason-Pfizer monkey virus

Method: X-Ray Diffraction

Resolution: 1.63 Å

See all 6 structures...

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Conserved folding in retroviral proteases: crystal structure of a synthetic HIV-1 protease.

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