EMBO J. 1990 May;9(5):1511-7.

Translocation of bFGF to the nucleus is G1 phase cell cycle specific in bovine aortic endothelial cells.

Baldin V, Roman AM, Bosc-Bierne I, Amalric F, Bouche G.

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Centre de Recherche de Biochimie et de Genetique Cellulaires, CNRS, Toulouse, France.

Abstract

Primary cultures of adult bovine aortic endothelial (ABAE) cells require bFGF to grow. G1-arrested cells, obtained after 48 h without serum and bFGF, were found to enter S phase and grow synchronously for at least two generations on addition of bFGF. In growing cells bFGF was detected both in the cytoplasm (90%) and in the nucleus (10%) where it accumulates in the nucleolus. It was not detected in the nucleus of confluent cells. bFGF uptake was continuous in the cytoplasm throughout the cell cycle with a maximum in G2, while nuclear uptake occurred only in late G1. Cytoplasmic bFGF (18.4 kd) is cleaved into a 16.5 kd peptide in G1 (t1/2 = 30 min). In the nucleus the 18.4 kd form was the only one detected 2 h following bFGF addition and was then cleaved into the 16.5 kd in early S phase. These results are consistent with the possibility that in addition to the classical pathway of signal transduction, bFGF is directly translocated to the nucleus in late G1, and could play a role in replication and/or in transcription of rDNA.

PMID:: 2328724; [PubMed - indexed for MEDLINE]
PMCID:: PMC551843

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