Exp Gerontol. 1990;25(3-4):383-91.

Adenosine system and cell calcium translocation: interference of calcium channel blockers.

Laghi Pasini F, Capecchi PL, Pasqui AL, Ceccatelli L, Mazza S, Gistri A, Di Perri T.

Source

Istituto di Patologia Medica, University of Siena, Italy.

Abstract

Adenosine is able to inhibit in vitro neutrophil functions induced by formyl-methionyl-leucyl-phenylalanine (FMLP) and A23187, but not phorbol 12-myristate 13-acetate (PMA). The inhibiting activity on A23187 is reversed by increasing extracellular Ca2++ concentration. The calcium entry blocker flunarizine shows an activity very similar to that of adenosine. Both adenosine and flunarizine prevent Ca++ influx into activated neutrophils as detected by the fluorescent Ca++ chelator Quin-2. Finally, flunarizine binds to the neutrophil membrane and adenosine competitively inhibits flunarizine binding as assessed by 1H-Nuclear Magnetic Resonance (1H-NMR) technique, thus indicating that the two agents share a common binding site on the cell membrane.

PMID:: 2226674; [PubMed - indexed for MEDLINE]

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