Interactive exploration of bivariate data from a selected region of interest (ROI) in the image (a). For the ROI (a), the user selects two image channels (here the CD44 channel and the EpCam channel) and one tool, e.g. a scatter plot (b) from the visualization tab (see figure 3). The pixel values corresponding to the same location within the ROI are displayed as points in the scatterplot. Selection of points
in the plot (c) triggers highlighting the referring pixels in the image (displayed as red regions superimposing on the original image), with respect to the following criterion:
, with Γ escribing the selection of points
in the scatter plot,
and
defines the minimum and maximum of the selection range regarding EpCam values and
is the EpCam value of point
. The same applies to the CD44 values, accordingly. This process is often referred to as "gating" or "link-and-brush".