FEMS Microbiol Lett. 1990 Jul;58(2):221-5.

High efficiency electroporation of Pseudomonas aeruginosa using frozen cell suspensions.

Source

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.

Abstract

High efficiency transformation of Pseudomonas aeruginosa was achieved using frozen cell suspensions and high voltage electroporation. We have obtained frequencies as high as 5.8 x 10(8) transformants/micrograms of plasmid DNA using PA01 strain OT684 and a buffer of 15% glycerol-1 mM MOPS. The method allows for easy and reproducible production of frozen cell suspensions for rapid transformation of P. aeruginosa.

PMID:: 2121587; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

DNA, Bacterial

LinkOut - more resources

Other Literature Sources

COS Scholar Universe

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Transformation of Pseudomonas aeruginosa by electroporation. [Anal Biochem. 1990]
Transformation of Pseudomonas aeruginosa by electroporation.
Diver JM, Bryan LE, Sokol PA. Anal Biochem. 1990 Aug 15; 189(1):75-9.
Transformation of Burkholderia pseudomallei by electroporation. [Anal Biochem. 1996]
Transformation of Burkholderia pseudomallei by electroporation.
Mack K, Titball RW. Anal Biochem. 1996 Nov 1; 242(1):73-6.
A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation. [J Microbiol Methods. 2006]
A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation.
Choi KH, Kumar A, Schweizer HP. J Microbiol Methods. 2006 Mar; 64(3):391-7. Epub 2005 Jun 28.
[The study of optimal conditions of electroporation in Pseudomonas aeruginosa]. [Yi Chuan Xue Bao. 2004]
[The study of optimal conditions of electroporation in Pseudomonas aeruginosa].
Shan ZY, Xu HJ, Shi XQ, Nie Z, Yu Y, Zhang XM, Bai YL, Qiao MQ, Gao CC. Yi Chuan Xue Bao. 2004 Mar; 31(3):311-6.
Transformation of Pseudomonas aeruginosa strain PAO with bacteriophage and plasmid DNA. [Aust J Biol Sci. 1978]
Transformation of Pseudomonas aeruginosa strain PAO with bacteriophage and plasmid DNA.
Sinclair MI, Morgan AF. Aust J Biol Sci. 1978 Dec; 31(6):679-88.

See reviews... See all...

Cited by 23 PubMed Central articles

Transcriptomic analysis reveals a global alkyl-quinolone-independent regulatory role for PqsE in facilitating the environmental adaptation of Pseudomonas aeruginosa to plant and animal hosts. [Environ Microbiol. 2010]
Transcriptomic analysis reveals a global alkyl-quinolone-independent regulatory role for PqsE in facilitating the environmental adaptation of Pseudomonas aeruginosa to plant and animal hosts.
Rampioni G, Pustelny C, Fletcher MP, Wright VJ, Bruce M, Rumbaugh KP, Heeb S, Cámara M, Williams P. Environ Microbiol. 2010 Jun; 12(6):1659-73. Epub 2010 Apr 7.
GacA-controlled activation of promoters for small RNA genes in Pseudomonas fluorescens. [Appl Environ Microbiol. 2010]
GacA-controlled activation of promoters for small RNA genes in Pseudomonas fluorescens.
Humair B, Wackwitz B, Haas D. Appl Environ Microbiol. 2010 Mar; 76(5):1497-506. Epub 2010 Jan 4.
Stereospecificity of the siderophore pyochelin outer membrane transporters in fluorescent pseudomonads. [J Biol Chem. 2009]
Stereospecificity of the siderophore pyochelin outer membrane transporters in fluorescent pseudomonads.
Hoegy F, Lee X, Noel S, Rognan D, Mislin GL, Reimmann C, Schalk IJ. J Biol Chem. 2009 May 29; 284(22):14949-57. Epub 2009 Mar 17.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

High efficiency electroporation of Pseudomonas aeruginosa using frozen cell susp...
High efficiency electroporation of Pseudomonas aeruginosa using frozen cell suspensions.
FEMS Microbiol Lett. 1990 Jul ;58(2):221-5.

PubMed
Hepatic transcriptomic responses to TCDD in dioxin-sensitive and dioxin-resistan...
Hepatic transcriptomic responses to TCDD in dioxin-sensitive and dioxin-resistant rats during the onset of toxicity.
Toxicol Appl Pharmacol. 2011 Mar 1 ;251(2):119-29. Epub 2011 Jan 5 .

PubMed
The reported prevalence of urinary symptoms in women in one rural general practi...
The reported prevalence of urinary symptoms in women in one rural general practice.
Br J Gen Pract. 1990 Aug ;40(337):335-7.

PubMed
Prevalence and cumulative incidence of abnormal cervical cytology among HIV-infe...
Prevalence and cumulative incidence of abnormal cervical cytology among HIV-infected Thai women: a 5.5-year retrospective cohort study.
BMC Infect Dis. 2011 Jan 7 ;11:8.

PubMed
Genetic diversity of penicillin-binding protein 2 genes of penicillin-resistant ...
Genetic diversity of penicillin-binding protein 2 genes of penicillin-resistant strains of Neisseria meningitidis revealed by fingerprinting of amplified DNA.
Antimicrob Agents Chemother. 1990 Aug ;34(8):1523-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: