Cell. 1990 Oct 19;63(2):417-24.

A self-splicing group I intron in the DNA polymerase gene of Bacillus subtilis bacteriophage SPO1.

Goodrich-Blair H, Scarlato V, Gott JM, Xu MQ, Shub DA.

Source

Department of Biological Sciences, State University of New York, Albany 12222.

Abstract

We report a self-splicing intron in bacteriophage SPO1, whose host is the gram-positive Bacillus subtilis. The intron contains all the conserved features of primary sequence and secondary structure previously described for the group IA introns of eukaryotic organelles and the gram-negative bacteriophage T4. The SPO1 intron contains an open reading frame of 522 nucleotides. As in the T4 introns, this open reading frame begins in a region that is looped out of the secondary structure, but ends in a highly conserved region of the intron core. The exons encode SPO1 DNA polymerase, which is highly similar to E. coli DNA polymerase I. The demonstration of self-splicing introns in viruses of both gram-positive and gram-negative eubacteria lends further evidence for their early origin in evolution.

PMID:: 2119891; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Secondary Source ID, Grant Support

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

The DNA polymerase genes of several HMU-bacteriophages have similar group I introns with highly divergent open reading frames. [Nucleic Acids Res. 1994]
The DNA polymerase genes of several HMU-bacteriophages have similar group I introns with highly divergent open reading frames.
Goodrich-Blair H, Shub DA. Nucleic Acids Res. 1994 Sep 11; 22(18):3715-21.
The DNA polymerase-encoding gene of Bacillus subtilis bacteriophage SPO1. [Gene. 1992]
The DNA polymerase-encoding gene of Bacillus subtilis bacteriophage SPO1.
Scarlato V, Gargano S. Gene. 1992 Sep 1; 118(1):109-13.
An intron in the thymidylate synthase gene of Bacillus bacteriophage beta 22: evidence for independent evolution of a gene, its group I intron, and the intron open reading frame. [Proc Natl Acad Sci U S A. 1994]
An intron in the thymidylate synthase gene of Bacillus bacteriophage beta 22: evidence for independent evolution of a gene, its group I intron, and the intron open reading frame.
Bechhofer DH, Hue KK, Shub DA. Proc Natl Acad Sci U S A. 1994 Nov 22; 91(24):11669-73.
Review A unique group of self-splicing introns in bacteriophage T4. [Indian J Biochem Biophys. 2001]
Review A unique group of self-splicing introns in bacteriophage T4.
Khan AU, Ajamaluddin M, Ahmad M. Indian J Biochem Biophys. 2001 Oct; 38(5):289-93.
Review Group II introns and expression of conjugative transfer functions in lactic acid bacteria. [Antonie Van Leeuwenhoek. 1999]
Review Group II introns and expression of conjugative transfer functions in lactic acid bacteria.
Dunny GM, McKay LL. Antonie Van Leeuwenhoek. 1999 Jul-Nov; 76(1-4):77-88.

See reviews... See all...

Cited by 31 PubMed Central articles

Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease family. [Nucleic Acids Res. 2011]
Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease family.
Taylor GK, Heiter DF, Pietrokovski S, Stoddard BL. Nucleic Acids Res. 2011 Dec; 39(22):9705-19. Epub 2011 Sep 2.
Review Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity. [Nucleic Acids Res. 2011]
Review Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity.
Chan SH, Stoddard BL, Xu SY. Nucleic Acids Res. 2011 Jan; 39(1):1-18. Epub 2010 Aug 30.
Conservation of intron and intein insertion sites: implications for life histories of parasitic genetic elements. [BMC Evol Biol. 2009]
Conservation of intron and intein insertion sites: implications for life histories of parasitic genetic elements.
Swithers KS, Senejani AG, Fournier GP, Gogarten JP. BMC Evol Biol. 2009 Dec 31; 9:303. Epub 2009 Dec 31.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A self-splicing group I intron in the DNA polymerase gene of Bacillus subtilis b...
A self-splicing group I intron in the DNA polymerase gene of Bacillus subtilis bacteriophage SPO1.
Cell. 1990 Oct 19 ;63(2):417-24.

PubMed
Comparison of imputation methods for handling missing covariate data when fittin...
Comparison of imputation methods for handling missing covariate data when fitting a Cox proportional hazards model: a resampling study.
BMC Med Res Methodol. 2010 Dec 31 ;10:112.

PubMed
Correspondence in cancer history between husbands and wives.
Correspondence in cancer history between husbands and wives.
Jpn J Cancer Res. 1990 Jun-Jul ;81(6-7):584-9.

PubMed
Cluster analysis for identifying sub-groups and selecting potential discriminato...
Cluster analysis for identifying sub-groups and selecting potential discriminatory variables in human encephalitis.
BMC Infect Dis. 2010 Dec 31 ;10:364.

PubMed
Analysis of glass ionomer cement with use of scanning electron microscopy.
Analysis of glass ionomer cement with use of scanning electron microscopy.
J Prosthet Dent. 1990 Aug ;64(2):167-74.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

A self-splicing group I intron in the DNA polymerase gene of Bacillus subtilis bacteriophage SPO1.

Source

Abstract

Publication Types, MeSH Terms, Substances, Secondary Source ID, Grant Support

Publication Types

MeSH Terms

Substances

Secondary Source ID

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 31 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information