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    J Clin Microbiol. 1990 Jun;28(6):1451-3.

    Cord formation in BACTEC 7H12 medium for rapid, presumptive identification of Mycobacterium tuberculosis complex.

    Source

    Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester, New York 14642.

    Abstract

    We evaluated cord formation in BACTEC 7H12 medium as a criterion for rapid identification of Mycobacterium tuberculosis complex. Kinyoun-stained smears, prepared from 270 radiometrically positive BACTEC 7H12 bottles, were examined independently by three observers. Smears from 93.2, 88.6, and 83.0% of the M. tuberculosis complex cultures were read as cord positive, and smears from 97.3, 97.8, and 99.5% of the mycobacteria other than M. tuberculosis cultures were read as cord negative by the three observers, respectively. There was 93.3% agreement between the observers. The presence of cords in BACTEC 7H12 medium can be a reliable criterion for rapid, presumptive identification of M. tuberculosis complex.

    PMID:
    2116448
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC267954
    Free PMC Article

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