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    J Mol Signal. 2010 Aug 13;5:13.

    UV-B induced transcript accumulation of DAHP synthase in suspension-cultured Catharanthus roseus cells.

    Ramani S, Patil N, Jayabaskaran C.

    Source

    Department of Biochemistry, Indian Institute of Science, Bangalore-560012, India. cjb@biochem.iisc.ernet.in.

    Abstract

    The enzyme 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase (EC 4.1.2.15) catalyzes the first committed step in the shikimate pathway of tryptophan synthesis, an important precursor for the production of terpenoid indole alkaloids (TIAs). A full-length cDNA encoding nuclear coded chloroplast-specific DAHP synthase transcript was isolated from a Catharanthus roseus cDNA library. This had high sequence similarity with other members of plant DAHP synthase family. This transcript accumulated in suspension cultured C. roseus cells on ultraviolet (UV-B) irradiation. Pretreatment of C.roseus cells with variety of agents such as suramin, N-acetyl cysteine, and inhibitors of calcium fluxes and protein kinases and MAP kinase prevented this effect of UV-B irriadiation. These data further show that the essential components of the signaling pathway involved in accumulation DAHP synthase transcript in C. roseus cells include suramin-sensitive cell surface receptor, staurosporine-sensitive protein kinase and MAP kinase.

    PMID:
    20704760
    [PubMed]
    PMCID: PMC2930624
    Free PMC Article

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