Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5729-33.

A phorbol ester/diacylglycerol-binding protein encoded by the unc-13 gene of Caenorhabditis elegans.

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Medical Research Council Molecular Genetics Unit, Cambridge, United Kingdom.

Abstract

Mutations in the unc-13 gene cause diverse defects in the nervous system of the nematode Caenorhabditis elegans. Molecular cloning of the gene and sequencing of the cDNA revealed that the product encodes a protein, 1734 amino acids in length, with a central domain with sequence similarity to the regulatory region of protein kinase C. The domain was expressed in Escherichia coli and shown to bind specifically to a phorbol ester in the presence of calcium; diacylglycerol inhibited the binding in a competitive manner. These findings confirm that the unc-13 gene product has binding sites similar to those of protein kinase C and may be a component of an alternative transduction pathway of the diacylglycerol signal to a different effector function in the nervous system.

PMID:: 2062851; [PubMed - indexed for MEDLINE]
PMCID:: PMC51951

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The Caenorhabditis elegans unc-13 gene product is a phospholipid-dependent high-affinity phorbol ester receptor. [Biochem J. 1992]
The Caenorhabditis elegans unc-13 gene product is a phospholipid-dependent high-affinity phorbol ester receptor.
Ahmed S, Maruyama IN, Kozma R, Lee J, Brenner S, Lim L. Biochem J. 1992 Nov 1; 287 ( Pt 3):995-9.
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Rozengurt E, Sinnett-Smith J, Van Lint J, Valverde AM. Mutat Res. 1995 Dec; 333(1-2):153-60.

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A phorbol ester/diacylglycerol-binding protein encoded by the unc-13 gene of Caenorhabditis elegans.

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