BMC Bioinformatics. 2010 Jul 5;11:371.

Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing.

Source

Pfizer Global Research and Development, Sandwich, UK. david-olivier.azulay@pfizer.com

Abstract

BACKGROUND:

OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality.

RESULTS:

We can assess performance characteristics of OFFGEL IEF peptide fractionation in proteomics by generating plots of the overall fractionation patterns and the pairwise comparisons of adjacent fractions.

CONCLUSIONS:

A visualisation tool for peptide fractionation has been developed to support the evaluation of IEF data quality and can be implemented in proteomics research.

PMID:: 20602788; [PubMed - indexed for MEDLINE]
PMCID: PMC2908104

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