Nucleic Acids Res. 2010 Jul;38(Web Server issue):W348-51. Epub 2010 Jun 15.

rQuant.web: a tool for RNA-Seq-based transcript quantitation.

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Friedrich Miescher Laboratory of the Max Planck Society, Tübingen, Germany. regina.bohnert@tuebingen.mpg.de

Abstract

We provide a novel web service, called rQuant.web, allowing convenient access to tools for quantitative analysis of RNA sequencing data. The underlying quantitation technique rQuant is based on quadratic programming and estimates different biases induced by library preparation, sequencing and read mapping. It can tackle multiple transcripts per gene locus and is therefore particularly well suited to quantify alternative transcripts. rQuant.web is available as a tool in a Galaxy installation at http://galaxy.fml.mpg.de. Using rQuant.web is free of charge, it is open to all users, and there is no login requirement.

PMID:: 20551130; [PubMed - indexed for MEDLINE]
PMCID:: PMC2896134

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