Trends Biochem Sci. 1991 Jan;16(1):5-10.

13C-NMR: a simple yet comprehensive method for analysis of intermediary metabolism.

Jeffrey FM, Rajagopal A, Malloy CR, Sherry AD.

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Mary Nell and Ralph B. Rogers Magnetic Resonance Center, Department of Radiology, Dallas 75235-8573.

Abstract

13C-NMR is a particularly attractive tool for metabolic studies because of its inherent simplicity: all labeled products at sufficient concentration may be identified and analysed in a single spectrum. However, the real power behind the approach presented here is the ability to measure groups of individual 13C-isotopomers (isotope isomers). The information that this provides is superior to conventional tracer techniques, allowing a very detailed description of metabolic events. Several examples are given of the value of this convenient and straightforward analysis for some problems of current interest in intermediary metabolism.

PMID:: 2053137; [PubMed - indexed for MEDLINE]

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