Phylogentic analysis of the FtsZ-tubulin superfamily and representative operonic architectures of FtsZl1 family genes. Color code: eukaryotes - blue; bacteria - green; archaea - orange. The RELL bootstrap values (%) are indicated for selected major branches. A. Phylogeny of the FtsZ-tubulin superfamily. The tree was reconstructed from 118 phylogenetically informative positions. B. Phylogeny of FtsZl1 family. The tree was reconstructed from 103 phylogenetically informative positions. C. Genomic context of the FtsZl1 family genes. Homologous genes are shown by arrows of the same color; genes are shown not to scale. Color code for the gene context: green arrows, VWA-domain-containing proteins; red, FtsZ-like 1 family proteins; brown, OmpA-like proteins; pale blue, serine/threonine protein kinases; yellow, GTPase; empty arrows (A1-A2; B1-B9) represent families that are associated with the FtsZ-like 1 genes in many genomes (see Additional File 6); gray arrows, genes associated with the FtsZ-like 1 family genes in one or a few related genomes.

Multiple alignment of conserved blocks of all previously described and the two new protein families of the FtsZ-tubulin superfamily. The sequences are denoted by their GI numbers and species names. The positions of the first and the last residues of the aligned region in the corresponding protein are indicated for each sequence. Key elements of secondary structure based on known FtsZ tertiary structures are shown on two lines above the alignment [6,12,44]. The evolutionarily conserved functional motifs of the FtsZ and tubulin families are shown underneath the alignment for the corresponding groups [8,9]. The alignment columns are colored on the basis of the respective position conservation throughout the superfamily: yellow background indicates hydrophobic residues (ACFILMVWY), red letters indicate polar residues (DEHKNQR), blue letters indicate hydroxyl-containing residues (TS), and green background indicates small residues (ACGNPSTV). Species abbreviations: Methu - Methanospirillum hungatei JF-1; Magma - Magnetospirillum magneticum AMB-1; Deira - Deinococcus radiodurans R1; Plepa - Plesiocystis pacifica SIR-1; Bacca - Bacteroides caccae ATCC 43185; Bacbr - Brevibacillus brevis NBRC 100599; Clokl - Clostridium kluyveri DSM 555; Polna - Polaromonas naphthalenivorans; Glovi - Gloeobacter violaceus PCC 7421; Micch - Microcoleus chthonoplastes; Nosto - Nostoc sp. PCC 7120; Metin - Methylacidiphilum infernorum V4; Theel - Thermosynechococcus elongatus BP-1; Chlag - Chloroflexus aggregans DSM 9485; Metac - Methanosarcina acetivorans C2A; Pyrab - Pyrococcus abyssi GE5; Halut - Halorhabdus utahensis DSM 12940; Rhoba - Rhodopirellula baltica; Blama - Blastopirellula marina DSM 3645; Chrvi - Chromobacterium violaceum; Marin - Marinomonas sp.; Psefl - Pseudomonas fluorescens; BaccH - Bacillus cereus H3081-97; Bacce - Bacillus cereus 03BB102; Metja - Methanocaldococcus jannaschii; Korcr - Korarchaeum cryptofilum OPF8; Naneq - Nanoarchaeum equitans Kin4-M; Myctu - Mycobacterium tuberculosis H37Rv; Aquae - Aquifex aeolicus VF5; Thema - Thermotoga maritima MSB8; Escco - Escherichia coli K-12; Theth - Thermus thermophilus HB8; Sacce - Saccharomyces cerevisiae.