Biochemistry. 1991 Apr 9;30(14):3576-82.

Mechanistic stoichiometry of mitochondrial oxidative phosphorylation.

Hinkle PC, Kumar MA, Resetar A, Harris DL.

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Section of Biochemistry, Molecular, and Cell Biology, Cornell University, Ithaca, New York 14853.

Abstract

P/O ratios of rat liver mitochondria were measured with particular attention to systematic errors. Corrections for energy loss during oxidative phosphorylation were made by measurement of respiration as a function of mitochondrial membrane potential. The corrected values were close to 1, 0.5, and 1 at the three coupling sites, respectively. These values are consistent with recent measurements of mitochondrial proton transport.

PMID:: 2012815; [PubMed - indexed for MEDLINE]

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