Cell. 1991 Mar 22;64(6):1123-34.

DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein.

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Research Institute for Molecular Pathology, Vienna, Austria.

Abstract

We have studied the mechanism by which DNA methylation inhibits transcription both in cell-free nuclear extracts and in the living cell. Repression of transcription in vitro for four different promoters was shown to be an indirect effect. The mediator of repression had properties indistinguishable from those of a methyl-CpG binding protein (MeCP-1) that has been previously identified. Use of differentially methylated promoters and methylated competitors in transient transfection assays suggested that indirect repression via MeCP-1 also occurs in the living cell. This was supported by the fact that MeCP-1-deficient cells showed much reduced repression of methylated genes.

PMID:: 2004419; [PubMed - indexed for MEDLINE]

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Cited by over 100 PubMed Central articles

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Structures reported by this article

The Solution Structure Of The Domain From Mecp2 That Binds To Methylated Dna

PDB: 1QK9

Source: Homo sapiens

Method: Solution Nmr

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