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Genome Biol. 2009;10(12):R142. Epub 2009 Dec 22.

A blind deconvolution approach to high-resolution mapping of transcription factor binding sites from ChIP-seq data.

Lun DS, Sherrid A, Weiner B, Sherman DR, Galagan JE.

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Phenomics and Bioinformatics Research Centre, School of Mathematics and Statistics, and Australian Centre for Plant Functional Genomics, University of South Australia, Mawson Lakes Boulevard, Mawson Lakes, SA 5095, Australia. desmond.lun@unisa.edu.au

Abstract

We present CSDeconv, a computational method that determines locations of transcription factor binding from ChIP-seq data. CSDeconv differs from prior methods in that it uses a blind deconvolution approach that allows closely-spaced binding sites to be called accurately. We apply CSDeconv to novel ChIP-seq data for DosR binding in Mycobacterium tuberculosis and to existing data for GABP in humans and show that it can discriminate binding sites separated by as few as 40 bp.

PMID:: 20028542; [PubMed - indexed for MEDLINE]
PMCID: PMC2812949

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