PLoS Pathog. 2009 Aug;5(8):e1000554. Epub 2009 Aug 21.

CpG methylation controls reactivation of HIV from latency.

Blazkova J, Trejbalova K, Gondois-Rey F, Halfon P, Philibert P, Guiguen A, Verdin E, Olive D, Van Lint C, Hejnar J, Hirsch I.

Source

Institut National de la Santé et de la Recherche Médicale (INSERM), UMR891, Centre de Recherche en Cancérologie de Marseille; and Institut Paoli-Calmettes, Marseille, France.

Abstract

DNA methylation of retroviral promoters and enhancers localized in the provirus 5' long terminal repeat (LTR) is considered to be a mechanism of transcriptional suppression that allows retroviruses to evade host immune responses and antiretroviral drugs. However, the role of DNA methylation in the control of HIV-1 latency has never been unambiguously demonstrated, in contrast to the apparent importance of transcriptional interference and chromatin structure, and has never been studied in HIV-1-infected patients. Here, we show in an in vitro model of reactivable latency and in a latent reservoir of HIV-1-infected patients that CpG methylation of the HIV-1 5' LTR is an additional epigenetic restriction mechanism, which controls resistance of latent HIV-1 to reactivation signals and thus determines the stability of the HIV-1 latency. CpG methylation acts as a late event during establishment of HIV-1 latency and is not required for the initial provirus silencing. Indeed, the latent reservoir of some aviremic patients contained high proportions of the non-methylated 5' LTR. The latency controlled solely by transcriptional interference and by chromatin-dependent mechanisms in the absence of significant promoter DNA methylation tends to be leaky and easily reactivable. In the latent reservoir of HIV-1-infected individuals without detectable plasma viremia, we found HIV-1 promoters and enhancers to be hypermethylated and resistant to reactivation, as opposed to the hypomethylated 5' LTR in viremic patients. However, even dense methylation of the HIV-1 5'LTR did not confer complete resistance to reactivation of latent HIV-1 with some histone deacetylase inhibitors, protein kinase C agonists, TNF-alpha, and their combinations with 5-aza-2deoxycytidine: the densely methylated HIV-1 promoter was most efficiently reactivated in virtual absence of T cell activation by suberoylanilide hydroxamic acid. Tight but incomplete control of HIV-1 latency by CpG methylation might have important implications for strategies aimed at eradicating HIV-1 infection.

PMID:: 19696893; [PubMed - indexed for MEDLINE]
PMCID:: PMC2722084

Free PMC Article

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Figure 2

Negative correlation of CpG density in the 5′ LTR and reactivation of HIV-1 provirus.

(A) Flow chart protocol showing two consecutive cycles of 24-h stimulation of H12 cells with TNF-α and PMA, followed by the first and second cell sorting of EGFP-negative cells. Negative sortings are separated by 24-day and followed by 60-day cultivation periods in the absence of any exogeneous stimulator to reach steady-state culture conditions. In the frames are shown the percentages of EGFP-negative cells determined at the moment of cell sorting and the percentages of methylated CpGs in HIV-1 promoter determined in the EGFP-negative cell population just after cell separation. (B) Reactivation levels of HIV-1 promoters in clones harboring latent HIV-1. The percentages of EGFP⁺ cells in cell clones exposed to TNF-α (10 ng/ml) and PMA (10 nM) for 24 h are shown. The cell clones were prepared by sublimit dilution of the population of H12 cells 60 days after the second sorting of EGFP-negative cells (as shown in flow chart protocol, panel A, and in Figure S1, panel I) and analyzed after the outgrowth of cell clones 20 days later. The cell culture from which the clones were derived was analyzed under the same conditions, 20 days after cell cloning (H12, 2×EGFP⁻). (C,D) Immunofluorescence of EGFP and CD69 in 1B6 clone stimulated with TNF-α (10 ng/ml) and PMA (10 nM) for 24 h (D) and in non-stimulated control (C). (E) CpG methylation profile of the 5′ LTR in non-stimulated 1B6 clone and in 1B6 clone stimulated for 24 h. (F,G) Immunofluorescence of EGFP and CD69 in 2D12 clone stimulated with TNF-α (10 ng/ml) and PMA (10 nM) for 24 h (G) and in non-stimulated control (F). (H) CpG methylation profile of the 5′ LTR in non-stimulated 2D12 clone and in 2D12 clone stimulated for 24 h. (I) Negative correlation of CpG density in the 5′ LTR of HIV-1 provirus with EGFP expression in latently infected Jurkat clonal cell lines exposed to TNF-α and PMA. Open circles, nonmethylated CpG residues; closed circles, methylated CpG residues (mCpG).

CpG Methylation Controls Reactivation of HIV from Latency

PLoS Pathog. PLoS Pathog;5(8):e1000554.

Figure 6

Methylation analysis of HIV-1 promoter in memory CD4⁺ T cells purified from HIV-1-infected individuals.

Hypermethylation of the HIV-1 5′ LTR in HIV-1-infected long-term aviremic individuals contrasts with hypomethylation of the HIV-1 5′ LTR in viremic patients and negatively correlates with reactivation of the HIV-1 provirus. (A) CpG methylation patterns in the bisulfite-treated HIV-1 5′ LTR sequences of aviremic patients were clustered using neighbor-joining method into several groups within each patient (separated by horizontal bars) with a strong bootstrap support (>990/1000). The sequences within each group differed in addition in about 1 to 10 point-mutations. Note that the variability of bisulfite-treated sequences is underestimated due to the conversion of the majority of cytosine residues to thymine (except for cytosine in 5-methylcytosine residues). Open circles, nonmethylated CpG residues; closed circles, methylated CpG residues. (B) Percentage of methylated CpGs (mCpG) in the 5′ LTR of HIV-1 in long-term aviremic (n = 6) and viremic (n = 7) patients. p, non-parametric, two side Mann-Whitney test. (C–F) Reactivation of HIV-1 provirus in memory CD4⁺ T cells obtained from patients with different levels of CpG methylation. Memory CD4⁺ T cells cultured for three days in the presence of reactivating agents were 10-fold serially diluted in duplicate and co-cultured with PHA-activated CD4⁺ T cells from an allogeneic healthy donor. HIV-1 replication was followed by determination of p24 in the cell-free supernatant. Percentage of reactivable provirus is presented as a ratio of end-point dilutions of patients' CD4⁺ T cells producing HIV-1 virus to the number of DNA proviral copies in the cell quantity equivalent to end-point dilution and determined by quantitative PCR, as indicated in Table 1. (C) Non-stimulated CD4⁺ T cells. (D) CD4⁺ T cells stimulated with TNF-α at 10 ng/ml and 10-nM PMA. (E) CD4⁺ T cells stimulated with 500-nM TSA and 10-nM PMA. (F) CD4⁺ T cells stimulated with 5-µM 5-aza-dC. (C–F) Open symbols, viremic patients; closed symbols, aviremic patients.

CpG Methylation Controls Reactivation of HIV from Latency

PLoS Pathog. PLoS Pathog;5(8):e1000554.