Matrix showing the expression strength for a set of genes related to brain (columns), for a fixed Theiler stage (TS28). A structure filtered statistical analysis has been carried out to select only six structures. The bars on the left show the color code employed.

(A) Matrix showing the expression strength during the development for a fixed gene (Pax6). Pax6 is a transcriptional factor involved in the development of central nervous system and retina. A structure filtered statistical analysis has been carried out to, selecting only those structures related to CNS and eye. Observe the similar expression pattern for retina, hindbrain and eye during the developmental stages comprised between TS20 and TS22 (B). The information displayed in the structure filtered matrix shown in A can be summarized in the structure correlation matrix shown here. The color code ranges from red to blue. Red color indicates the maximum expression pattern correlation along the development for a given gene in two different structures. Here, maximum correlations can be found for example between eye and hindbrain, between hindbrain and retina, and between retina and eye. (C) From the structure correlation matrix in B, the system can infer several comparative matrices showing the expression pattern during the 27 developmental stages of the correlated anatomical components. These comparative matrices highlight the similar expression pattern of Pax6 in central nervous system and eye by filtering by P-value.

(A) Matrix showing the gene-expression level for a set of genes during the development for a fixed anatomical structure (in this case, brain). The matrix is displayed following a color code gradient (below the matrix) that depends on the gene-expression strength value. There are two bars below the matrix. The horizontal colored bar denotes the expression level color code that ranges from red (strong expression) to blue (weak expression). The white color on the vertical bar represents the lack of available data; the grey color denotes the inexistence of the anatomical structure under study in a particular developmental stage and the black color means no expression. (B) Gene correlation matrix. The information displayed in the gene matrix shown in A can be summarized in the gene correlation matrix shown here. The color code for matrix elements is shown on right hand side of the matrix. The result is a color gradient correlation matrix in which genes with similar expression patterns (strongly co-expressed) are represented by red positions and the cyan positions correspond to genes with opposed expression patterns (negative correlated genes). (C) From the gene correlation matrix in B, the system can infer several comparative matrices showing the expression pattern during the 27 developmental stages of the correlated genes. Observe the similar expression pattern of genes Mirn124a-1 and Mirn124a-2.