S Afr Med J. 1991 Nov 16;80(10):515-6.

The use of the polymerase chain reaction test in the diagnosis of tuberculosis.

du van Helden PD, Toit R, Jordaan A, Taljaard B, Pitout J, Victor T.

Source

Department of Medical Biochemistry, University of Stellenbosch.

Abstract

Current techniques for laboratory diagnosis of tuberculosis have some serious limitations. These include the high cost and time required for the current assays. The development of a rapid, sensitive, specific and low-cost assay is therefore of considerable importance. We report here the development and laboratory testing of a polymerase chain reaction DNA-based diagnostic test for the presence of Mycobacterium tuberculosis in sputum. The assay shows a high level of sensitivity and specificity and requires considerably less capital, consumables and time inputs than existing laboratory tests. We believe this technology is ready for large-scale evaluation and use, particularly in hospital-based laboratories.

PMID:: 1948472; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms

Publication Types

Comparative Study

MeSH Terms

LinkOut - more resources

Medical

Tuberculosis - MedlinePlus Health Information

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

[Mycobacterial tests]. [Kekkaku. 2008]
[Mycobacterial tests].
Takashima T, Higuchi T. Kekkaku. 2008 Jan; 83(1):43-59.
A refined symptom-based approach to diagnose pulmonary tuberculosis in children. [Pediatrics. 2006]
A refined symptom-based approach to diagnose pulmonary tuberculosis in children.
Marais BJ, Gie RP, Hesseling AC, Schaaf HS, Lombard C, Enarson DA, Beyers N. Pediatrics. 2006 Nov; 118(5):e1350-9.
[Problems in the standardization of the polymerase chain reaction for the diagnosis of pulmonary tuberculosis]. [Rev Saude Publica. 1999]
[Problems in the standardization of the polymerase chain reaction for the diagnosis of pulmonary tuberculosis].
Bollela VR, Sato DN, Fonseca BA. Rev Saude Publica. 1999 Jun; 33(3):281-6.
Review Diagnosis of tuberculosis: available technologies, limitations, and possibilities. [J Clin Lab Anal. 2003]
Review Diagnosis of tuberculosis: available technologies, limitations, and possibilities.
Garg SK, Tiwari RP, Tiwari D, Singh R, Malhotra D, Ramnani VK, Prasad GB, Chandra R, Fraziano M, Colizzi V, et al. J Clin Lab Anal. 2003; 17(5):155-63.
Review Use of an insertion sequence for laboratory diagnosis and epidemiologic studies of tuberculosis. [Ann Emerg Med. 1994]
Review Use of an insertion sequence for laboratory diagnosis and epidemiologic studies of tuberculosis.
Eisenach KD. Ann Emerg Med. 1994 Sep; 24(3):450-3.

See reviews... See all...

Cited by 2 PubMed Central articles

In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression. [BMC Microbiol. 2005]
In-house nucleic acid amplification tests for the detection of Mycobacterium tuberculosis in sputum specimens: meta-analysis and meta-regression.
Flores LL, Pai M, Colford JM Jr, Riley LW. BMC Microbiol. 2005 Oct 3; 5:55. Epub 2005 Oct 3.
Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory. [J Clin Microbiol. 1993]
Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory.
Clarridge JE 3rd, Shawar RM, Shinnick TM, Plikaytis BB. J Clin Microbiol. 1993 Aug; 31(8):2049-56.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The use of the polymerase chain reaction test in the diagnosis of tuberculosis.
The use of the polymerase chain reaction test in the diagnosis of tuberculosis.
S Afr Med J. 1991 Nov 16 ;80(10):515-6.

PubMed
Suppression of N-myc downstream-regulated gene 2 is associated with induction of...
Suppression of N-myc downstream-regulated gene 2 is associated with induction of Myc in colorectal cancer and correlates closely with differentiation.
Biol Pharm Bull. 2009 Jun ;32(6):968-75.

PubMed
Cloning and expression of a cocaine-sensitive rat dopamine transporter.
Cloning and expression of a cocaine-sensitive rat dopamine transporter.
Science. 1991 Oct 25 ;254(5031):578-9.

PubMed
Considerations for assessing the severity of an influenza pandemic.
Considerations for assessing the severity of an influenza pandemic.
Wkly Epidemiol Rec. 2009 May 29 ;84(22):197-202.

PubMed
Measurement and reliability: statistical thinking considerations.
Measurement and reliability: statistical thinking considerations.
Schizophr Bull. 1991 ;17(3):483-9.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: