Science. 1991 Oct 25;254(5031):539-44.

X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel coiled coil.

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Howard Hughes Medical Institute, Cambridge, MA 02142.

Abstract

The x-ray crystal structure of a peptide corresponding to the leucine zipper of the yeast transcriptional activator GCN4 has been determined at 1.8 angstrom resolution. The peptide forms a parallel, two-stranded coiled coil of alpha helices packed as in the "knobs-into-holes" model proposed by Crick in 1953. Contacts between the helices include ion pairs and an extensive hydrophobic interface that contains a distinctive hydrogen bond. The conserved leucines, like the residues in the alternate hydrophobic repeat, make side-to-side interactions (as in a handshake) in every other layer of the dimer interface. The crystal structure of the GCN4 leucine zipper suggests a key role for the leucine repeat, but also shows how other features of the coiled coil contribute to dimer formation.

PMID:: 1948029; [PubMed - indexed for MEDLINE]

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Structures reported by this article

Gcn4-Leucine Zipper Core Mutant As N16a Trigonal Automatic Solution

PDB: 3K7Z

Source: Saccharomyces cerevisiae

Method: X-Ray Diffraction

Resolution: 1.9 Å

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X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel coiled coil.

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