Nucleic Acids Res. 1991 Nov 11;19(21):6027-31.

A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium.

Peterson SN, Schramm N, Hu PC, Bott KF, Hutchison CA 3rd.

Source

Curriculum in Genetics, University of North Carolina, Chapel Hill 27599.

Abstract

A physical map of the Mycoplasma genitalium genome has been prepared using pulsed-field gel electrophoresis. This report details recent efforts made to add markers or specific loci to this map in the absence of any mutants or system of genetic exchange. A total of 44 random clones were partially sequenced. Computer analysis was performed in an attempt to identify homologies with genes already recorded in the DNA sequence database. Clones with a large extent of homology to genes from other microorganisms have been assigned to specific loci on the M. genitalium map by hybridization to selected restriction digests. The additional data has facilitated an updated version of the physical map, and verified this random sequencing method as a useful mapping procedure as well as offering new insight into the physiological processes of this fastidious organism.

PMID:: 1945886; [PubMed - indexed for MEDLINE]
PMCID:: PMC329062

Free PMC Article

Publication Types, MeSH Terms, Substances, Secondary Source ID, Grant Support

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Research Support, U.S. Gov't, P.H.S.

MeSH Terms

Substances

Genetic Markers

Secondary Source ID

GENBANK/X61510
GENBANK/X61511
GENBANK/X61512
GENBANK/X61513
GENBANK/X61514
GENBANK/X61515
GENBANK/X61516
GENBANK/X61517
GENBANK/X61518
GENBANK/X61519
GENBANK/X61520
GENBANK/X61521
GENBANK/X61522
GENBANK/X61523
GENBANK/X61524
GENBANK/X61525
GENBANK/X61526
GENBANK/X61527
GENBANK/X61528
GENBANK/X61529
GENBANK/X61530
GENBANK/X61531
GENBANK/X61532
GENBANK/X61533
GENBANK/X61534
GENBANK/X61535
GENBANK/X61536
GENBANK/X61537
GENBANK/X61538
GENBANK/X61539

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Related citations in PubMed

A survey of the Mycoplasma genitalium genome by using random sequencing. [J Bacteriol. 1993]
A survey of the Mycoplasma genitalium genome by using random sequencing.
Peterson SN, Hu PC, Bott KF, Hutchison CA 3rd. J Bacteriol. 1993 Dec; 175(24):7918-30.
A physical map of the Mycoplasma genitalium genome. [Mol Microbiol. 1990]
A physical map of the Mycoplasma genitalium genome.
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Review Physical mapping of bacterial genomes.
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Cited by 8 PubMed Central articles

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A survey of the Mycoplasma genitalium genome by using random sequencing. [J Bacteriol. 1993]
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