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    Clin Chem. 1977 May;23(5):882-4.

    Cholesterol determination in high-density lipoproteins separated by three different methods.

    Lopes-Virella MF, Stone P, Ellis S, Colwell JA.

    Abstract

    We describe a simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low-density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+. Values so obtained correlate well with values obtained with the heparin-Mn2+ precipitation technique (r = 0.95, CV less than 5% in 66% of the subjects studied and between 5 and 10% in the remaining ones) or by ultracentrifugal separation (r = 0.82, CV less than 5% in 80% of the subjects studied and between 5 and 10% in the remaining ones). Our precipitation technique is more appropriate for routine clinical laboratory use.

    PMID:
    192488
    [PubMed - indexed for MEDLINE]
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