Proc Natl Acad Sci U S A. 1991 May 15;88(10):4363-6.

Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces.

Kang AS, Barbas CF, Janda KD, Benkovic SJ, Lerner RA.

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Department of Molecular Biology Scripps Research Institute, La Jolla, CA 92037.

Abstract

We describe a method based on a phagemid vector with helper phage rescue for the construction and rapid analysis of combinatorial antibody Fab libraries. This approach should allow the generation and selection of many monoclonal antibodies. Antibody genes are expressed in concert with phage morphogenesis, thereby allowing incorporation of functional Fab molecules along the surface of filamentous phage. The power of the method depends upon the linkage of recognition and replication functions and is not limited to antibody molecules.

PMID:: 1903540; [PubMed - indexed for MEDLINE]
PMCID:: PMC51659

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