Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82.

Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.

Barbas CF 3rd, Kang AS, Lerner RA, Benkovic SJ.

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Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.

Abstract

A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

PMID:: 1896445; [PubMed - indexed for MEDLINE]
PMCID:: PMC52428

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