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    Gene. 1991 Jun 15;102(1):39-44.

    Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase.

    Arthington BA, Bennett LG, Skatrud PL, Guynn CJ, Barbuch RJ, Ulbright CE, Bard M.

    Source

    Department of Biology, Indiana University-Purdue University at Indianapolis 46205.

    Abstract

    The ERG3 gene from Saccharomyces cerevisiae has been cloned by complementation of an erg3-2 mutation. ERG3 is the putative gene encoding the C-5 sterol desaturase required for ergosterol biosynthesis. The functional gene has been localized on a 2.5-kb HindIII-BamHI fragment containing an open reading frame comprising 365 amino acids. Gene disruption resulting from a deletion/substitution demonstrates that ERG3 is not essential for cell viability or the sparking function.

    PMID:
    1864507
    [PubMed - indexed for MEDLINE]

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