EMBO J. 1991 Feb;10(2):449-57.

Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames.

Source

Cold Spring Harbor Laboratory, Cold Spring Harbor 11724.

Abstract

Bovine papillomavirus (BPV) DNA is maintained as an episome with a constant copy number in transformed cells and is stably inherited. To study BPV replication we have developed a transient replication assay based on a highly efficient electroporation procedure. Using this assay we have determined that in the context of the viral genome two of the viral open reading frames, E1 and E2, are required for replication. Furthermore we show that when produced from expression vectors in the absence of other viral gene products, the full length E2 transactivator polypeptide and a 72 kd polypeptide encoded by the E1 open reading frame in its entirety, are both necessary and sufficient for replication BPV in C127 cells.

PMID:: 1846806; [PubMed - indexed for MEDLINE]
PMCID:: PMC452666

Free PMC Article

LinkOut - more resources

Full Text Sources

Other Literature Sources

Labome Researcher Resource - ExactAntigen/Labome

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Bovine papillomavirus with a mutation in the E2 serine 301 phosphorylation site replicates at a high copy number. [J Virol. 1991]
Bovine papillomavirus with a mutation in the E2 serine 301 phosphorylation site replicates at a high copy number.
McBride AA, Howley PM. J Virol. 1991 Dec; 65(12):6528-34.
Identification of a 68-kilodalton nuclear ATP-binding phosphoprotein encoded by bovine papillomavirus type 1. [J Virol. 1990]
Identification of a 68-kilodalton nuclear ATP-binding phosphoprotein encoded by bovine papillomavirus type 1.
Sun S, Thorner L, Lentz M, MacPherson P, Botchan M. J Virol. 1990 Oct; 64(10):5093-105.
Bovine papillomavirus type 1 E1 and simian virus 40 large T antigen share regions of sequence similarity required for multiple functions. [J Virol. 1997]
Bovine papillomavirus type 1 E1 and simian virus 40 large T antigen share regions of sequence similarity required for multiple functions.
Mansky KC, Batiza A, Lambert PF. J Virol. 1997 Oct; 71(10):7600-8.
Review The bovine papillomavirus replicon. [Ciba Found Symp. 1986]
Review The bovine papillomavirus replicon.
Botchan M, Berg L, Reynolds J, Lusky M. Ciba Found Symp. 1986; 120:53-67.
Review Papillomavirus transforming functions. [Ciba Found Symp. 1986]
Review Papillomavirus transforming functions.
Howley PM, Yang YC, Spalholz BA, Rabson MS. Ciba Found Symp. 1986; 120:39-52.

See reviews... See all...

Cited by over 100 PubMed Central articles

Development of a cellular assay system to study the genome replication of high- and low-risk mucosal and cutaneous human papillomaviruses. [J Virol. 2011]
Development of a cellular assay system to study the genome replication of high- and low-risk mucosal and cutaneous human papillomaviruses.
Geimanen J, Isok-Paas H, Pipitch R, Salk K, Laos T, Orav M, Reinson T, Ustav M Jr, Ustav M, Ustav E. J Virol. 2011 Apr; 85(7):3315-29. Epub 2011 Jan 19.
Effective formation of the segregation-competent complex determines successful partitioning of the bovine papillomavirus genome during cell division. [J Virol. 2010]
Effective formation of the segregation-competent complex determines successful partitioning of the bovine papillomavirus genome during cell division.
Silla T, Männik A, Ustav M. J Virol. 2010 Nov; 84(21):11175-88. Epub 2010 Sep 1.
Viral trans-factor independent replication of human papillomavirus genomes. [Virol J. 2010]
Viral trans-factor independent replication of human papillomavirus genomes.
Pittayakhajonwut D, Angeletti PC. Virol J. 2010 Jun 10; 7:123. Epub 2010 Jun 10.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Protein Clusters
Clusters of related proteins from the Protein Clusters database that cite the current articles. Sources of references in Protein Clusters include the associated Gene and Conserved Domain records as well as NCBI added citations.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Transient replication of BPV-1 requires two viral polypeptides encoded by the E1...
Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames.
EMBO J. 1991 Feb ;10(2):449-57.

PubMed
Reliability of the Life Satisfaction Questionnaire to assess patients with chron...
Reliability of the Life Satisfaction Questionnaire to assess patients with chronic musculoskeletal pain.
Int J Rehabil Res. 2008 Jun ;31(2):181-3.

PubMed
Differentiation between the intensity of breathlessness and the distress it evok...
Differentiation between the intensity of breathlessness and the distress it evokes in normal subjects during exercise.
Clin Sci (Lond). 1991 Jan ;80(1):65-70.

PubMed
Cell system ontology: representation for modeling, visualizing, and simulating b...
Cell system ontology: representation for modeling, visualizing, and simulating biological pathways.
In Silico Biol. 2007 ;7(6):623-38.

PubMed
The myoD gene family: nodal point during specification of the muscle cell lineag...
The myoD gene family: nodal point during specification of the muscle cell lineage.
Science. 1991 Feb 15 ;251(4995):761-6.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames.

Source

Abstract

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by over 100 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information