J Bacteriol. 1991 Aug;173(16):5220-3.

A novel activity in Escherichia coli K-12 that directs restriction of DNA modified at CG dinucleotides.

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New England Biolabs, Beverly, Massachusetts 01915.

Abstract

The restriction systems McrA and McrB of Escherichia coli K-12 are known to attack DNA containing modified cytosine. In strains lacking both activities, however, we observed that DNA methylated at CG dinucleotides (as is mammalian DNA) was still significantly restricted. We show that this substantial barrier to the acceptance of 5-methylcytosine-containing DNA is attributable to a hitherto unknown activity of the Mrr restriction system. Strikingly, the multiple systems used by this gut inhabitant to determine the fate of invading DNA will all limit genetic exchange with its mammalian host(s), reinforcing the idea that one role of DNA methylation is to serve as a "molecular passport" (E. A. Raleigh, R. Trimarchi, and H. Revel, Genetics 122:279-296, 1989).

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PMCID:: PMC208216

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Escherichia coli K-12 restricts DNA containing 5-methylcytosine. [Proc Natl Acad Sci U S A. 1986]
Escherichia coli K-12 restricts DNA containing 5-methylcytosine.
Raleigh EA, Wilson G. Proc Natl Acad Sci U S A. 1986 Dec; 83(23):9070-4.
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Cited by 18 PubMed Central articles

Review Conflicts targeting epigenetic systems and their resolution by cell death: novel concepts for methyl-specific and other restriction systems. [DNA Res. 2010]
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Mulligan EA, Hatchwell E, McCorkle SR, Dunn JJ. Nucleic Acids Res. 2010 Apr; 38(6):1997-2005. Epub 2009 Dec 16.
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