Nat Methods. 2008 Mar;5(3):235-7. Epub 2008 Feb 10.

Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.

Hamady M, Walker JJ, Harris JK, Gold NJ, Knight R.

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Department of Computer Science, UCB 430, University of Colorado, Boulder, Colorado 80309, USA.

Abstract

We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.

PMID:: 18264105; [PubMed - indexed for MEDLINE]

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