Science. 1987 Jun 5;236(4806):1299-302.

Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes.

Abstract

A variant of the cauliflower mosaic virus 35S promoter with transcriptional activity approximately tenfold higher than that of the natural promoter was constructed by tandem duplication of 250 base pairs of upstream sequences. The duplicated region also acted as a strong enhancer of heterologous promoters, increasing the activity of an adjacent and divergently transcribed transferred DNA gene several hundredfold, and to a lesser extent, that of another transferred DNA gene from a remote downstream position. This optimized enhancer element should be very useful for obtaining high levels of expression of foreign genes in transgenic plants.

PMID:: 17770331; [PubMed]

LinkOut - more resources

Full Text Sources

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Bi-directional duplex promoters with duplicated enhancers significantly increase transgene expression in grape and tobacco. [Transgenic Res. 2004]
Bi-directional duplex promoters with duplicated enhancers significantly increase transgene expression in grape and tobacco.
Li ZT, Jayasankar S, Gray DJ. Transgenic Res. 2004 Apr; 13(2):143-54.
Regulated expression of genes encoding soybean beta-conglycinins in transgenic plants. [Dev Genet. 1989]
Regulated expression of genes encoding soybean beta-conglycinins in transgenic plants.
Chen ZL, Naito S, Nakamura I, Beachy RN. Dev Genet. 1989; 10(2):112-22.
Characteristics of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter. [Plant Mol Biol. 1990]
Characteristics of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter.
Sanger M, Daubert S, Goodman RM. Plant Mol Biol. 1990 Mar; 14(3):433-43.
Localization of Elements Important for the Wound-Inducible Expression of a Chimeric Potato Proteinase Inhibitor II-CAT Gene in Transgenic Tobacco Plants. [Plant Cell. 1990]
Localization of Elements Important for the Wound-Inducible Expression of a Chimeric Potato Proteinase Inhibitor II-CAT Gene in Transgenic Tobacco Plants.
Keil M, Sanchez-Serrano J, Schell J, Willmitzer L. Plant Cell. 1990 Jan; 2(1):61-70.
Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants. [Plant Cell Physiol. 1996]
Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants.
Mitsuhara I, Ugaki M, Hirochika H, Ohshima M, Murakami T, Gotoh Y, Katayose Y, Nakamura S, Honkura R, Nishimiya S, et al. Plant Cell Physiol. 1996 Jan; 37(1):49-59.

See reviews... See all...

Cited by 67 PubMed Central articles

Isolation and functional characterization of a cotton ubiquitination-related promoter and 5'UTR that drives high levels of expression in root and flower tissues. [BMC Biotechnol. 2011]
Isolation and functional characterization of a cotton ubiquitination-related promoter and 5'UTR that drives high levels of expression in root and flower tissues.
Viana AA, Fragoso RR, Guimarães LM, Pontes N, Oliveira-Neto OB, Artico S, Nardeli SM, Alves-Ferreira M, Batista JA, Silva MC, et al. BMC Biotechnol. 2011 Nov 24; 11:115. Epub 2011 Nov 24.
Toward a general model for the evolutionary dynamics of gene duplicates. [Genome Biol Evol. 2011]
Toward a general model for the evolutionary dynamics of gene duplicates.
Konrad A, Teufel AI, Grahnen JA, Liberles DA. Genome Biol Evol. 2011; 3:1197-209. Epub 2011 Sep 13.
Visualization of plant viral suppressor silencing activity in intact leaf lamina by quantitative fluorescent imaging. [Plant Methods. 2011]
Visualization of plant viral suppressor silencing activity in intact leaf lamina by quantitative fluorescent imaging.
Stephan D, Slabber C, George G, Ninov V, Francis KP, Burger JT. Plant Methods. 2011 Aug 3; 7:25. Epub 2011 Aug 3.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant G...
Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes.
Science. 1987 Jun 5 ;236(4806):1299-302.

PubMed
[Influence of patient's personal characteristics on anxiety level, oral hygiene,...
[Influence of patient's personal characteristics on anxiety level, oral hygiene, pain intensity at infiltrative anesthesia puncture and time of professional care].
Av Odontoestomatol. 1991 Sep ;7(7):519-21.

PubMed
Determining the burden of tuberculosis in Eritrea: a new approach.
Determining the burden of tuberculosis in Eritrea: a new approach.
Bull World Health Organ. 2007 Aug ;85(8):593-9.

PubMed
Synonymous codon usage in Saccharomyces cerevisiae.
Synonymous codon usage in Saccharomyces cerevisiae.
Yeast. 1991 Oct ;7(7):657-78.

PubMed
Dual-association of gnotobiotic IL-10-/- mice with 2 nonpathogenic commensal bac...
Dual-association of gnotobiotic IL-10-/- mice with 2 nonpathogenic commensal bacteria induces aggressive pancolitis.
Inflamm Bowel Dis. 2007 Dec ;13(12):1457-66.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes.

Abstract

LinkOut - more resources

Full Text Sources

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 67 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information