J Mol Biol. 1992 Jan 5;223(1):17-22.

Minigene rescues acetylcholinesterase lethal mutations in Drosophila melanogaster.

Source

Department of Zoology and Animal Biology, University of Geneva, Switzerland.

Abstract

The gene encoding acetylcholinesterase in Drosophila melanogaster is over 34,000 base-pairs long. We have constructed a 5800 base-pair minigene containing 1500 base-pairs of genomic sequence upstream from the transcription start spliced to the coding sequence, but lacking the nine introns. After germline genetic transformation, this minigene rescues acetylcholinesterase lethal mutants. Tissue-specific distribution appears normal. This allows us to test site-directed mutations of acetylcholinesterase. In a first effort, deletion of most of the unusual 1000 bases leader and its intriguing short open reading frames showed no effect on gene expression. The way is open to study in vivo the structure-function relationships of acetylcholinesterase and insecticide resistance.

PMID:: 1731068; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

FlyBase

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Drosophila melanogaster acetylcholinesterase gene. Structure, evolution and mutations. [J Mol Biol. 1989]
Drosophila melanogaster acetylcholinesterase gene. Structure, evolution and mutations.
Fournier D, Karch F, Bride JM, Hall LM, Bergé JB, Spierer P. J Mol Biol. 1989 Nov 5; 210(1):15-22.
The Ace locus of Drosophila melanogaster: structural gene for acetylcholinesterase with an unusual 5' leader. [EMBO J. 1986]
The Ace locus of Drosophila melanogaster: structural gene for acetylcholinesterase with an unusual 5' leader.
Hall LM, Spierer P. EMBO J. 1986 Nov; 5(11):2949-54.
Structure and tissue-specific expression of the Drosophila melanogaster organellar-type Ca(2+)-ATPase gene. [Biochem J. 1995]
Structure and tissue-specific expression of the Drosophila melanogaster organellar-type Ca(2+)-ATPase gene.
Magyar A, Bakos E, Váradi A. Biochem J. 1995 Sep 15; 310 ( Pt 3):757-63.
Structure and promoter activity of the 5' flanking region of ace-1, the gene encoding acetylcholinesterase of class A in Caenorhabditis elegans. [J Mol Biol. 1999]
Structure and promoter activity of the 5' flanking region of ace-1, the gene encoding acetylcholinesterase of class A in Caenorhabditis elegans.
Culetto E, Combes D, Fedon Y, Roig A, Toutant JP, Arpagaus M. J Mol Biol. 1999 Jul 30; 290(5):951-66.
Promoter elements and transcriptional control of the mouse acetylcholinesterase gene. [J Biol Chem. 1993]
Promoter elements and transcriptional control of the mouse acetylcholinesterase gene.
Li Y, Camp S, Rachinsky TL, Bongiorno C, Taylor P. J Biol Chem. 1993 Feb 15; 268(5):3563-72.

See reviews... See all...

Cited by 4 PubMed Central articles

Costs of insensitive acetylcholinesterase insecticide resistance for the malaria vector Anopheles gambiae homozygous for the G119S mutation. [Malar J. 2010]
Costs of insensitive acetylcholinesterase insecticide resistance for the malaria vector Anopheles gambiae homozygous for the G119S mutation.
Djogbénou L, Noel V, Agnew P. Malar J. 2010 Jan 13; 9:12. Epub 2010 Jan 13.
A novel acetylcholinesterase gene in mosquitoes codes for the insecticide target and is non-homologous to the ace gene in Drosophila. [Proc Biol Sci. 2002]
A novel acetylcholinesterase gene in mosquitoes codes for the insecticide target and is non-homologous to the ace gene in Drosophila.
Weill M, Fort P, Berthomieu A, Dubois MP, Pasteur N, Raymond M. Proc Biol Sci. 2002 Oct 7; 269(1504):2007-16.
Replacement of the glycoinositol phospholipid anchor of Drosophila acetylcholinesterase with a transmembrane domain does not alter sorting in neurons and epithelia but results in behavioral defects. [Mol Biol Cell. 1996]
Replacement of the glycoinositol phospholipid anchor of Drosophila acetylcholinesterase with a transmembrane domain does not alter sorting in neurons and epithelia but results in behavioral defects.
Incardona JP, Rosenberry TL. Mol Biol Cell. 1996 Apr; 7(4):613-30.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Minigene rescues acetylcholinesterase lethal mutations in Drosophila melanogaste...
Minigene rescues acetylcholinesterase lethal mutations in Drosophila melanogaster.
J Mol Biol. 1992 Jan 5 ;223(1):17-22.

PubMed
Amyloid induced suicidal erythrocyte death.
Amyloid induced suicidal erythrocyte death.
Cell Physiol Biochem. 2007 ;19(1-4):175-84.

PubMed
Localisation of hypoxanthine phosphoribosyl transferase in the malaria parasite ...
Localisation of hypoxanthine phosphoribosyl transferase in the malaria parasite Plasmodium falciparum.
Exp Parasitol. 1992 Feb ;74(1):11-9.

PubMed
Time-dependent spectral analysis of epidemiological time-series with wavelets.
Time-dependent spectral analysis of epidemiological time-series with wavelets.
J R Soc Interface. 2007 Aug 22 ;4(15):625-36.

PubMed
Inhibition by chloroquine of a novel haem polymerase enzyme activity in malaria ...
Inhibition by chloroquine of a novel haem polymerase enzyme activity in malaria trophozoites.
Nature. 1992 Jan 9 ;355(6356):167-9.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: