J Neurochem. 1992 Feb;58(2):548-53.

Affinity purification of human tau proteins and the construction of a sensitive sandwich enzyme-linked immunosorbent assay for human tau detection.

Mercken M, Vandermeeren M, Lübke U, Six J, Boons J, Vanmechelen E, Van de Voorde A, Gheuens J.

Source

Innogenetics, Ghent, Belgium.

Abstract

Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau protein was used to purify tau proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau proteins. The affinity-purified human tau was used to produce a high-titered rabbit anti-human tau serum. The monoclonal anti-tau antibody and the polyclonal rabbit anti-tau serum were then used to construct a sandwich enzyme-linked immunosorbent assay for detection of human tau proteins, with a sensitivity of 1 ng/ml.

PMID:: 1729400; [PubMed - indexed for MEDLINE]

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