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Institut für Entwieklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Düsseldorf, Germany.
We have isolated and characterized cDNA clones for the leaf-specific C4-phosphoenolpyruvate carboxylase (PEPCase) from the dicotyledonous C4 plant Flaveria trinervia. The isolation of multiple cDNAs indicates that in this plant the C4 isoform is encoded by a small subgroup of the PEPCase gene family. The deduced amino acid sequence reveals a higher degree of similarity to the CAM and C3 isozymes of the dicotyledonous, facultative CAM plant Mesembryanthemum crystallinum than to the C4 PEPCases of monocotyledonous origin.
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