Exp Cell Res. 1991 Sep;196(1):137-40.

Organization of multiple nucleoids and DNA molecules in mitochondria of a human cell.

Source

University of Tsukuba, Institute of Biological Sciences, Ibaraki, Japan.

Abstract

Mitochondrial nucleoids (mt-nucleoids) of the A2780 line of cultured human cells were stained with DAPI and observed using an epifluorescence microscope. The mt-nucleoids appeared to be organized compactly in mitochondria. Numbers of mt-nucleoids per mitochondrion ranged from 1 to more than 10, and 70% were "multinucleated" mitochondria. Intensities of fluorescence of mt-nucleoids in each mitochondrion were measured by a video-intensified microscope system (VIM system) and copy numbers of mitochondrial DNA (mtDNA) in each mitochondria were determined. The copy numbers of mtDNA per mitochondrion ranged from 1 to 15, and the average was 4.6. Because the cells had 107 mitochondria on average, the copy number of mtDNA per cell was estimated to be about 500.

PMID:: 1715276; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

DNA, Mitochondrial

LinkOut - more resources

Full Text Sources

Elsevier Science

Medical

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Isolation of morphologically intact mitochondrial nucleoids from the yeast, Saccharomyces cerevisiae. [J Cell Sci. 1987]
Isolation of morphologically intact mitochondrial nucleoids from the yeast, Saccharomyces cerevisiae.
Miyakawa I, Sando N, Kawano S, Nakamura S, Kuroiwa T. J Cell Sci. 1987 Nov; 88 ( Pt 4):431-9.
Fluorescence microscopic studies on mitochondria and mitochondrial nucleoids in a wild-type strain and respiratory mutants of Candida albicans. [J Med Vet Mycol. 1988]
Fluorescence microscopic studies on mitochondria and mitochondrial nucleoids in a wild-type strain and respiratory mutants of Candida albicans.
Ito-Kuwa S, Aoki S, Watanabe T, Ehara T, Osafune T. J Med Vet Mycol. 1988; 26(4):207-17.
Fluorescence microscopic studies of mitochondrial nucleoids during meiosis and sporulation in the yeast, Saccharomyces cerevisiae. [J Cell Sci. 1984]
Fluorescence microscopic studies of mitochondrial nucleoids during meiosis and sporulation in the yeast, Saccharomyces cerevisiae.
Miyakawa I, Aoi H, Sando N, Kuroiwa T. J Cell Sci. 1984 Mar; 66:21-38.
Review [Morphology and organization of yeast mitochondrial nucleoids]. [Tanpakushitsu Kakusan Koso. 1994]
Review [Morphology and organization of yeast mitochondrial nucleoids].
Miyakawa I, Sando N. Tanpakushitsu Kakusan Koso. 1994 Mar; 39(4):601-10.
Review Molecular and cellular mechanisms of mitochondrial nuclear division and mitochondriokinesis. [Microsc Res Tech. 1994]
Review Molecular and cellular mechanisms of mitochondrial nuclear division and mitochondriokinesis.
Kuroiwa T, Ohta T, Kuroiwa H, Shigeyuki K. Microsc Res Tech. 1994 Feb 15; 27(3):220-32.

See reviews... See all...

Cited by 25 PubMed Central articles

Maximizing signal-to-noise ratio in the random mutation capture assay. [Nucleic Acids Res. 2012]
Maximizing signal-to-noise ratio in the random mutation capture assay.
Poovathingal SK, Gruber J, Ng LF, Halliwell B, Gunawan R. Nucleic Acids Res. 2012 Mar; 40(5):e35. Epub 2011 Dec 17.
Superresolution fluorescence imaging of mitochondrial nucleoids reveals their spatial range, limits, and membrane interaction. [Mol Cell Biol. 2011]
Superresolution fluorescence imaging of mitochondrial nucleoids reveals their spatial range, limits, and membrane interaction.
Brown TA, Tkachuk AN, Shtengel G, Kopek BG, Bogenhagen DF, Hess HF, Clayton DA. Mol Cell Biol. 2011 Dec; 31(24):4994-5010. Epub 2011 Oct 17.
Review Mitochondrial DNA mutations in disease and aging. [J Cell Biol. 2011]
Review Mitochondrial DNA mutations in disease and aging.
Park CB, Larsson NG. J Cell Biol. 2011 May 30; 193(5):809-18. Epub 2011 May 23.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

Organization of multiple nucleoids and DNA molecules in mitochondria of a human ...
Organization of multiple nucleoids and DNA molecules in mitochondria of a human cell.
Exp Cell Res. 1991 Sep ;196(1):137-40.

PubMed
Recruiting older people into a large, community-based study of heart failure.
Recruiting older people into a large, community-based study of heart failure.
Chronic Illn. 2005 Dec ;1(4):321-9.

PubMed
Should we resuscitate? Ethical dilemmas.
Should we resuscitate? Ethical dilemmas.
Ann Trop Paediatr. 1991 ;11(2):169-74.

PubMed
Auraptene decreases the activity of matrix metalloproteinases in dextran sulfate...
Auraptene decreases the activity of matrix metalloproteinases in dextran sulfate sodium-induced ulcerative colitis in ICR mice.
Biosci Biotechnol Biochem. 2006 Dec ;70(12):3062-5. Epub 2006 Dec 7 .

PubMed
Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast.
Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast.
Science. 1991 Aug 23 ;253(5022):905-9.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: