J Virol. 1991 Sep;65(9):5088-91.

The vpx gene of simian immunodeficiency virus facilitates efficient viral replication in fresh lymphocytes and macrophage.

Source

Department of Cancer Biology, Harvard School of Public Health, Boston, Massachusetts 02115.

Abstract

vpx is a unique open reading frame found in simian immunodeficiency virus (SIV) and human immunodeficiency virus type 2 (HIV-2) but not in HIV-1. It encodes a 12- to 16-kDa virion-associated protein. Although vpx is dispensable for viral replication in several established human lymphocyte cell lines, there is no consensus regarding whether this gene is required for efficient viral replication in freshly isolated lymphocytes. We report here that the vpx mutant of SIVmac exhibits different degrees of impairment from wild-type SIVmac in freshly isolated lymphocytes. This defect is more pronounced in macrophages from the same donors. Our findings suggest that vpx is required for efficient viral replication in fresh lymphocytes and macrophages.

PMID:: 1714525; [PubMed - indexed for MEDLINE]
PMCID:: PMC248975

Free PMC Article

Publication Types, MeSH Terms, Substances, Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Libraries

LinkOut Holdings

Supplemental Content

Save items

Related citations in PubMed

Human immunodeficiency virus type 2 vpx protein augments viral infectivity. [Virology. 1991]
Human immunodeficiency virus type 2 vpx protein augments viral infectivity.
Kappes JC, Conway JA, Lee SW, Shaw GM, Hahn BH. Virology. 1991 Sep; 184(1):197-209.
Functional analysis of the simian immunodeficiency virus Vpx protein: identification of packaging determinants and a novel nuclear targeting domain. [J Virol. 2001]
Functional analysis of the simian immunodeficiency virus Vpx protein: identification of packaging determinants and a novel nuclear targeting domain.
Mahalingam S, Van Tine B, Santiago ML, Gao F, Shaw GM, Hahn BH. J Virol. 2001 Jan; 75(1):362-74.
Functional analysis of the vpx, vpr, and nef genes of simian immunodeficiency virus. [J Acquir Immune Defic Syndr Hu...]
Functional analysis of the vpx, vpr, and nef genes of simian immunodeficiency virus.
Park IW, Sodroski J. J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Apr 1; 8(4):335-44.
Review Limelight on two HIV/SIV accessory proteins in macrophage infection: is Vpx overshadowing Vpr? [Retrovirology. 2010]
Review Limelight on two HIV/SIV accessory proteins in macrophage infection: is Vpx overshadowing Vpr?
Ayinde D, Maudet C, Transy C, Margottin-Goguet F. Retrovirology. 2010 Apr 9; 7:35. Epub 2010 Apr 9.
Review HIV-1 replication. [Somat Cell Mol Genet. 2001]
Review HIV-1 replication.
Freed EO. Somat Cell Mol Genet. 2001 Nov; 26(1-6):13-33.

See reviews... See all...

Cited by 23 PubMed Central articles

HIV/simian immunodeficiency virus (SIV) accessory virulence factor Vpx loads the host cell restriction factor SAMHD1 onto the E3 ubiquitin ligase complex CRL4DCAF1. [J Biol Chem. 2012]
HIV/simian immunodeficiency virus (SIV) accessory virulence factor Vpx loads the host cell restriction factor SAMHD1 onto the E3 ubiquitin ligase complex CRL4DCAF1.
Ahn J, Hao C, Yan J, DeLucia M, Mehrens J, Wang C, Gronenborn AM, Skowronski J. J Biol Chem. 2012 Apr 6; 287(15):12550-8. Epub 2012 Feb 23.
SAMHD1-deficient CD14+ cells from individuals with Aicardi-Goutières syndrome are highly susceptible to HIV-1 infection. [PLoS Pathog. 2011]
SAMHD1-deficient CD14+ cells from individuals with Aicardi-Goutières syndrome are highly susceptible to HIV-1 infection.
Berger A, Sommer AF, Zwarg J, Hamdorf M, Welzel K, Esly N, Panitz S, Reuter A, Ramos I, Jatiani A, et al. PLoS Pathog. 2011 Dec; 7(12):e1002425. Epub 2011 Dec 8.
Cellular restriction factors of feline immunodeficiency virus. [Viruses. 2011]
Cellular restriction factors of feline immunodeficiency virus.
Zielonka J, Münk C. Viruses. 2011 Oct; 3(10):1986-2005. Epub 2011 Oct 21.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The vpx gene of simian immunodeficiency virus facilitates efficient viral replic...
The vpx gene of simian immunodeficiency virus facilitates efficient viral replication in fresh lymphocytes and macrophage.
J Virol. 1991 Sep ;65(9):5088-91.

PubMed
Kinetic studies of HIV-1 and HIV-2 envelope glycoprotein-mediated fusion.
Kinetic studies of HIV-1 and HIV-2 envelope glycoprotein-mediated fusion.
Retrovirology. 2006 Dec 4 ;3:90.

PubMed
Signal transduction by normal isoforms and W mutant variants of the Kit receptor...
Signal transduction by normal isoforms and W mutant variants of the Kit receptor tyrosine kinase.
EMBO J. 1991 Sep ;10(9):2451-9.

PubMed
Biochemical differentiation of APOBEC3F and APOBEC3G proteins associated with HI...
Biochemical differentiation of APOBEC3F and APOBEC3G proteins associated with HIV-1 life cycle.
J Biol Chem. 2007 Jan 19 ;282(3):1585-94. Epub 2006 Dec 1 .

PubMed
Proteolytic release and crystallization of the RNase H domain of human immunodef...
Proteolytic release and crystallization of the RNase H domain of human immunodeficiency virus type 1 reverse transcriptase.
J Biol Chem. 1991 Aug 5 ;266(22):14697-702.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

The vpx gene of simian immunodeficiency virus facilitates efficient viral replication in fresh lymphocytes and macrophage.

Source

Abstract

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Libraries

Supplemental Content

Save items

Related citations in PubMed

Cited by 23 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information