Science. 1991 Jul 12;253(5016):191-4.

Structural features that give rise to the unusual stability of RNA hairpins containing GNRA loops.

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Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.

Abstract

The most frequently occurring RNA hairpins in 16S and 23S ribosomal RNA contain a tetranucleotide loop that has a GNRA consensus sequence. The solution structures of the GCAA and GAAA hairpins have been determined by nuclear magnetic resonance spectroscopy. Both loops contain an unusual G-A base pair between the first and last residue in the loop, a hydrogen bond between a G base and a phosphate, extensive base stacking, and a hydrogen bond between a sugar 2'-end OH and a base. These interactions explain the high stability of these hairpins and the sequence requirements for the variant and invariant nucleotides in the GNRA tetranucleotide loop family.

PMID:: 1712983; [PubMed - indexed for MEDLINE]

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Structures reported by this article

Gcaa Rna Tetraloop, Nmr, 10 Structures

PDB: 1ZIH

Source: synthetic construct

Method: Solution Nmr

See all 3 structures...

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